Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/28278
Title: Isolation and identification of a new tetrodotoxin-producing bacterial species, Raoultella terrigena, from Hong Kong marine puffer fish Takifugu niphobles
Authors: Yu, VCH
Yu, PHF
Ho, KC
Lee, FWF
Keywords: Raoultella terrigena
Takifugu niphobles
Tetrodotoxin
TTX-producing bacteria
Issue Date: 2011
Publisher: Mdpi Ag
Source: Marine drugs, 2011, v. 9, no. 11, p. 2384-2396 How to cite?
Journal: Marine Drugs 
Abstract: Puffer fish, Takifugu niphobles, collected from the Hong Kong coastal waters were screened for tetrodotoxin-producing bacteria. A Gram-negative, non-acid-fast, non-sporing and rod shaped bacterial strain (designated as gutB01) was isolated from the intestine of the puffer fish and was shown to produce tetrodotoxin (TTX). Based on the Microbial Identification (MIDI) and 16S-23S rDNA internal transcribed spacer (ITS) phylogenetic analysis, the strain was identified as Raoultella terrigena. The TTX production ability of the strain was confirmed by mouse bioassay, ELISA and mass spectrometry (MALDI-TOF). Our results reiterate that the TTX found in puffer fish was likely produced by the associated bacteria and TTX are widely produced amongst a diversity of bacterial species.
URI: http://hdl.handle.net/10397/28278
DOI: 10.3390/md9112384
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