Please use this identifier to cite or link to this item:
|Title:||Urine 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), a specific marker of oxidative stress, using direct, isocratic LC–MS/MS : method evaluation and application in study of biological variation in healthy adults|
|Source:||Clinica chimica acta, 2 Mar. 2010, v. 411, no. 5-6, p. 416-422 How to cite?|
|Journal:||Clinica chimica acta|
|Abstract:||Background: Urine 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) is a specific biomarker of oxidative stress. We evaluated a modified LC–MS/MS assay for urine 8-oxodG and determined biological variation in healthy adults.|
Method: Untreated urine was injected into an isocratic LC–MS/MS system (positive-ion MRM mode). Urine 8-oxodG in 51 healthy volunteers was measured; within- and between-day variations in 23 healthy volunteers were investigated.
Results: Dose–response was linear to 452 nmol/l; limit of detection=2.3 nmol/l; within-run and between-run CVs were <3.0% and <4.7%, respectively; recovery=97%–101%; accuracy=97.7–103.5%. Urine 8-oxodG (median, mean [SD]): 1.70, 1.70[0.60]nmol/mmol creatinine (n=51). Men had higher (p=0.027) concentrations than women matched for age and body mass index: mean [SD]: 1.90[1.60]; n=26 vs. 1.50[0.55]; n=25. Within- and between-day variations were wide but random. No significant differences were seen overall across time-points within 1 day or at the same time-point across 5 consecutive days.
Conclusions: The method has advantages of speed and relative simplicity as it does not require sample pre-treatment for 8-oxodG extraction, the use of internal standard or gradient LC elution and has high linearity, specificity, precision and recovery. Biological variation in urine 8-oxodG is wide, but no within- or between-day differences at the group concentration were seen in healthy adults.
|Rights:||Clinica Chimica Acta © 2009 Elsevier B.V. The journal web site is located at http://www.sciencedirect.com.|
|Appears in Collections:||Journal/Magazine Article|
Show full item record
Files in This Item:
|Benzie Clin Chim Acta paper 8oxodG.pdf||Pre-published version||342.43 kB||Adobe PDF||View/Open|
Citations as of Jun 2, 2016
WEB OF SCIENCETM
Citations as of Oct 16, 2016
Checked on Oct 16, 2016
Checked on Oct 16, 2016
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.