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Title: | Urine 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), a specific marker of oxidative stress, using direct, isocratic LC–MS/MS : method evaluation and application in study of biological variation in healthy adults | Authors: | Lee, KF Chung, WY Benzie, IFF |
Issue Date: | 2-Mar-2010 | Source: | Clinica chimica acta, 2 Mar. 2010, v. 411, no. 5-6, p. 416-422 | Abstract: | Background: Urine 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) is a specific biomarker of oxidative stress. We evaluated a modified LC–MS/MS assay for urine 8-oxodG and determined biological variation in healthy adults. Method: Untreated urine was injected into an isocratic LC–MS/MS system (positive-ion MRM mode). Urine 8-oxodG in 51 healthy volunteers was measured; within- and between-day variations in 23 healthy volunteers were investigated. Results: Dose–response was linear to 452 nmol/l; limit of detection=2.3 nmol/l; within-run and between-run CVs were <3.0% and <4.7%, respectively; recovery=97%–101%; accuracy=97.7–103.5%. Urine 8-oxodG (median, mean [SD]): 1.70, 1.70[0.60]nmol/mmol creatinine (n=51). Men had higher (p=0.027) concentrations than women matched for age and body mass index: mean [SD]: 1.90[1.60]; n=26 vs. 1.50[0.55]; n=25. Within- and between-day variations were wide but random. No significant differences were seen overall across time-points within 1 day or at the same time-point across 5 consecutive days. Conclusions: The method has advantages of speed and relative simplicity as it does not require sample pre-treatment for 8-oxodG extraction, the use of internal standard or gradient LC elution and has high linearity, specificity, precision and recovery. Biological variation in urine 8-oxodG is wide, but no within- or between-day differences at the group concentration were seen in healthy adults. |
Keywords: | Oxidative stress 8-oxodG Biological variation LC–MS/MS |
Publisher: | Elsevier | Journal: | Clinica chimica acta | ISSN: | 0009-8981 | DOI: | 10.1016/j.cca.2009.12.013 | Rights: | Clinica Chimica Acta © 2009 Elsevier B.V. The journal web site is located at http://www.sciencedirect.com. |
Appears in Collections: | Journal/Magazine Article |
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File | Description | Size | Format | |
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Benzie Clin Chim Acta paper 8oxodG.pdf | Pre-published version | 342.43 kB | Adobe PDF | View/Open |
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