Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/13775
Title: Anti-inflammatory effects of lutein in retinal ischemic/hypoxic injury : in vivo and in vitro studies
Authors: Li, SY
Fung, FKC
Fu, ZJ
Wong, D
Chan, HHL 
Lo, ACY
Issue Date: 2012
Publisher: Assoc Research Vision Ophthalmology Inc
Source: Investigative ophthalmology & visual science, 2012, v. 53, no. 10, p. 5976-5984 How to cite?
Journal: Investigative Ophthalmology & Visual Science 
Abstract: PURPOSE. Lutein protects retinal neurons by its anti-oxidative and anti-apoptotic properties in ischemia/reperfusion (I/R) injury while its anti-inflammatory effects remain unknown. As Muller cells play a critical role in retinal inflammation, the effect of lutein on Muller cells was investigated in a murine model of I/R injury and a culture model of hypoxic damage. METHODS. Unilateral retinal I/R was induced by a blockade of internal carotid artery using the intraluminal method in mice. Ischemia was maintained for 2 hours followed by 22 hours of reperfusion, during which either lutein (0.2 mg/kg) or vehicle was administered. Flash electroretinogram (flash ERG) and glial fibrillary acidic protein (GFAP) activation were assessed. Lutein's effect on Muller cells was further evaluated in immortalized rat Muller cells (rMC-1) challenged with cobalt chloride-induced hypoxia. Levels of IL-1 beta, cyclooxygenase-2 (Cox-2), TNF alpha, and nuclear factor-NF-kappa-B (NF-kappa B) were examined by Western blot analysis. RESULTS. Lutein treatment minimized deterioration of b-wave/a-wave ratio and oscillatory potentials as well as inhibited up-regulation of GFAP in retinal I/R injury. In cultured Muller cells, lutein treatment increased cell viability and reduced level of nuclear NF-kappa B, IL-1 beta, and Cox-2, but not TNF alpha after hypoxic injury. CONCLUSIONS. Reduced gliosis in I/R retina was observed with lutein treatment, which may contribute to preserved retinal function. Less production of pro-inflammatory factors from Muller cells suggested an anti-inflammatory role of lutein in retinal ischemic/hypoxic injury. Together with our previous studies, our results suggest that lutein protected the retina from ischemic/hypoxic damage by its anti-oxidative, antiapoptotic, and anti-inflammatory properties.
URI: http://hdl.handle.net/10397/13775
ISSN: 0146-0404
DOI: 10.1167/iovs.12-10007
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

21
Last Week
0
Last month
3
Citations as of Apr 10, 2016

WEB OF SCIENCETM
Citations

29
Last Week
0
Last month
1
Citations as of May 18, 2017

Page view(s)

38
Last Week
3
Last month
Checked on May 21, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.