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http://hdl.handle.net/10397/117958
| Title: | Long single-stranded DNA with site-specific modifications to enable single-base-resolution epigenetics | Authors: | Zhu, C Xu, Y Hao, Z Zhuang, Y Xu, R Pan, Y Shi, J Li, Y Jiang, Y Wang, T Yang, YR Liu, H Han, D Dong, Y He, C Liu, D |
Issue Date: | 16-Jan-2026 | Source: | Angewandte chemie international edition, 16 Jan. 2026, v. 65, no. 3, e13078 | Abstract: | We report a method for synthesizing site-specifically modified long single-stranded DNA (ssDNA) with high purity and fidelity. With the successful synthesis of long ssDNA carrying different 5-methylcytosine (5mC) patterns, single-base-resolution analysis of 5mC epigenetic dynamics at specific genomic loci were enabled in mammalian cells. The results revealed that the density of 5mC sites synergistically correlated with their maintenance. This study opens new avenues for precise epigenetic manipulation and therapeutic applications. | Keywords: | 5-Methyl cytosine Epigenetics Long single-stranded DNA Single-base resolution Site-specific modifications |
Publisher: | Wiley-VCH Verlag GmbH & Co. KGaA | Journal: | Angewandte chemie international edition | ISSN: | 1433-7851 | EISSN: | 1521-3773 | DOI: | 10.1002/anie.202513078 |
| Appears in Collections: | Journal/Magazine Article |
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