Method of biosynthesizing tetrodotoxin

Abstract

The present invention relates methods for the biosynthesis of tetrodotoxin (TTX) involving the steps of obtaining a culture possessing one or more of a Vibrio species, such as through a seed culture/ inoculating the culture and tissue extract from a textrodotoxin-bearing organism in a fermenter medium, and isolating and purifying tetrodotoxin from said fermenter, resulting in a yield of TTX of about 0.5 g TTX/L in about 3 to 5 days, such TTX being at least 90% pure. 本发明涉及河豚毒素的生物合成方法，包括获取具有一种或多种弧菌的培养，如通过种菌培养；将弧菌培养和产生TTX有机体的组织提取物加入发酵器中的培养基中；和从所述发酵器分离纯化TTX。本发明的方法在约3-5天的时间得到大约0.5mg/L的TTX产出，TTX的纯度至少为90％。