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Title: Modified beta-lactamases and uses thereof
Other Title: 修饰的β-内酰胺酶及其应用
Authors: Wong, KY 
Leung, TYC 
Chan, PH 
Issue Date: 1-Dec-2010
Source: 中国专利 ZL 200480012656.X
Abstract: A tool that can screen bacteria for beta-lactamases against a panel of various antibiotics is desirable. A biosensor incorporating an indicator molecule into beta-lactamases may achieve this purpose, but it requires that the attached indicator molecule must not impair the binding affinity of the protein to a great extent to provide a higher sensitivity. A modified beta-lactamases with a residue on the ohm-loop or outside the ohm-loop but close to the active site of beta-lactamase being replaced by a reactive residue is developed in this invention.
希望有一种能针对一组不同的β-内酰胺类抗生素筛选细菌以鉴定β- 内酰胺酶的工具。一种将指示分子导入β-内酰胺酶而形成的生物传感器可达到该目的,但要求导入的指示分子基本上不会削弱蛋白质结合的亲和力,以保证较高的灵敏度。本发明研发了一种经修饰的β-内酰胺酶,该β-内酰胺酶的Ω环上或Ω环的外侧但接近其活性部位的残基被活性残基置换。
Publisher: 中华人民共和国国家知识产权局
Rights: 专利权人: The Hong Kong Polytechnic University.
Appears in Collections:Patent

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