Monoclonal antibodies capable of specifically recognizing various salmonella serotypes, preparation method, detection kit and application

Abstract

The invention discloses monoclonal antibodies capable of specifically recognizing various salmonella serotypes, a preparation method, a detection kit and an application. The two monoclonal antibodies can specifically recognize a plurality of salmonella serotypes, and the two monoclonal antibodies are respectively named as SP1 and SP3. The SP1 and SP3 antibodies provided by the invention can specifically recognize salmonella of various serotypes, and can realize real-time detection and analysis of the salmonella of various serotypes in combination with an ELISA (enzyme-linked immuno sorbent assay) technology. On one hand, the antibodies provided by the invention can be made into a drug which has relatively large potential and is used for resisting the salmonella, and on the other hand, the antibodies can be used for developing a series of methods capable of specifically detecting the salmonella, besides, the detection steps of food-borne salmonella can be simplified, salmonella pollution can be warned in an early warning manner, and important guarantee is provided for food safety. 本发明公开能特异性识别多种沙门氏菌血清型的单克隆抗体及制备方法、检测试剂盒与应用。两种能特异性识别多种沙门氏菌血清型的单克隆抗体，所述两种单克隆抗体分别命名为SP1和SP3。本发明中提供的SP1和SP3抗体能特异性识别多种血清型的沙门氏菌，并可结合ELISA技术，实现对多种血清型的沙门氏菌进行实时检测分析。本发明的抗体一方面可以制成具有较大潜力的、用来对抗沙门氏菌的药物，另一方面该抗体也可以用来开发出一系列能够特异性检测沙门氏菌的方法，同时也可以简化食源性沙门氏菌检测步骤，预警沙门氏菌污染，为食物安全提供重要保障。