Nanoprobe, preparation method and application thereof, and detection system for miRNAs of circulating tumor cells

Abstract

The invention belongs to the technical field of medicines and particularly relates to a nanoprobe, a preparation method and application thereof, and a detection system for miRNAs of circulating tumor cells. The invention provides a nano probe. The nano probe comprises a light absorption nano material, polyethylene glycol, a circulating tumor cell target and a fluorescent group modified miRNAs single-chain probe, wherein the circulating tumor cell target is connected with the light absorption nanometer material through the polyethylene glycol, and the fluorescent group modified miRNAs single-chain probe is absorbed on a surface of the light absorption nanometer material. According to the nano probe, the miRNAs of the CTCs can be sensitively detected in a single living CTC without enriching the CTCs, so technical defects that the CTCs are difficult to enrich and are easy to cause invasive damage to the CTCs in the existing miRNA method for detecting the CTCs are effectively overcome. 本申请属于医药技术领域，尤其涉及一种纳米探针及其制备方法和应用、循环肿瘤细胞的miRNAs的检测系统。本申请提供了一种纳米探针包括：光吸收纳米材料、聚乙二醇、循环肿瘤细胞靶向物和荧光基团修饰的miRNAs单链探针；所述循环肿瘤细胞靶向物通过所述聚乙二醇与所述光吸收纳米材料连接，所述荧光基团修饰的miRNAs单链探针吸附在所述光吸收纳米材料的表面。本申请无需富集CTCs即可在单个活CTC中灵敏地检测其miRNAs，有效解决现有检测CTCs的miRNA方法中存在的CTCs难以富集，容易对CTCs造成侵入性破坏的技术缺陷。