Double-layer osteochondral stent material as well as preparation method and application thereof

Abstract

The invention discloses a double-layer bone cartilage scaffold material as well as a preparation method and application thereof. The scaffold material comprises a cartilage phase and a subchondral bone phase, the PmLnDMA is used for forming the cartilage phase and can be subjected to photo-crosslinking; the PmLnDMA/MH nano composite material is used for forming the subchondral bone phase; wherein P is polypropylene glycol; m is the number of monomers in the polypropylene glycol; l is polylactic acid; n is the number of monomers in polylactic acid; dMA is dimethyl acrylate, and MH is methacrylic acid hydroxyapatite. The hydrophobic injectable underwater cross-linked double-layer osteochondral scaffold disclosed by the invention is quickly implemented in a liquid environment and is subjected to cross-linking injection to form a firm two-phase interface, cartilage generation and osteogenesis are synergistically enhanced by adjusting a physical and chemical microenvironment, and cell functions of mesenchymal stem cells can be synergistically influenced by mechanical stimulation and growth factor stimulation; in-vitro and in-vivo cartilage and osteogenic differentiation of the mesenchymal stem cells is regulated and controlled. 本发明公开了一种双层骨软骨支架材料及其制备方法与应用，所述支架材料包括软骨相和软骨下骨相；构成所述软骨相的可光交联的PmLnDMA；以及构成所述软骨下骨相的PmLnDMA/MH纳米复合材料；其中，P为聚丙二醇；m为聚丙二醇内单体的个数；L为聚乳酸；n为聚乳酸内单体的个数；DMA为二甲基丙烯酸酯，MH为甲基丙烯酸化羟基磷灰石。本发明的疏水性可注射的水下交联双层骨软骨支架，在液体环境中快速实施并交联注射并形成坚固的两相界面，通过调节物理化学微环境协同增强软骨生成和成骨，机械刺激和生长因子刺激可以协同影响间充质干细胞的细胞功能，以调控间充质干细胞在体外和体内的软骨和成骨分化。