Metabonomics research method of lignan compounds

Abstract

The invention belongs to the technical field of metabonomics, and particularly relates to a metabonomics research method of lignan compounds. The method comprises the following steps: preparing a lignan compound solution; mixing the lignan compound solution with the resuscitated intestinal flora solution, carrying out shaking table culture, and collecting a plurality of culture solution samples atdifferent culture times; carrying out centrifugal separation on the culture solution sample, taking a supernatant, carrying out liquid chromatography-mass spectrometry detection and data analysis, and determining a metabolite structure and a metabolic pathway of the lignan compound; and verifying the anti-osteoporosis activity effect of the metabolite of the lignan compound. The research method not only can effectively analyze the anti-osteoporosis activity effect of the metabolites of the lignan compounds, but also can select the high-yield culture time point of the metabolites to enrich themetabolites, and provides good application of the metabolites of the lignan compounds in the aspect of treating anti-osteoporosis. 本发明属于代谢组学技术领域，具体涉及一种木脂素类化合物的代谢组学研究方法，包括如下步骤：配制木脂素类化合物溶液；将所述木脂素类化合物溶液与复苏后的肠道菌群液混合进行摇床培养，采集不同培养时间的多个培养液样品；将所述培养液样品进行离心分离，取上清液进行液质联用色谱检测并进行数据分析，确定木脂素类化合物的代谢产物结构和代谢路径；验证所述木脂素类化合物的代谢产物抗骨质疏松活性作用。该研究方法不仅可以有效分析木脂素类化合物的代谢产物抗骨质疏松活性作用，而且能选择代谢产物高产的培养时间点对代谢产物进行富集，为木脂素类化合物代谢产物在治疗抗骨质疏松症方面提供很好的应用。