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|Title:||Effects of essence of chicken on immunological responses and biochemical changes in an animal model||Authors:||Yan, Chun-man||Degree:||M.Phil.||Issue Date:||2005||Abstract:||Aims This study investigated the effects of essence of chicken (EOC) on immunological responses, free radicals and blood glucose concentrations in normal and burn wound rat models. Methods This thesis reports an experimental study on Sprague-Dawley rats with a 3-group time-series design: a sham operated group (UNBURN) (n=72), a bum inflicted group (BURN) (n=72) and a no treatment group (CONTROL) (n=24). Rats in the UNBURN and BURN groups were further subdivided by intake of normal saline (NS), gelatin (GEL) and essence of chicken (EOC) (n=24 each group). Blood samples were taken on days 1, 8, 15 and 22 postbum to measure concentrations of serum immunoglobulin G (IgG), immunoglobulin A (IgA), immunoglobulin M (IgM), plasma nitric oxide (NO), lipid peroxidation (LPO) and blood glucose. ANOVA was used for statistical analysis and the level of significance was set at p<0.05. Results Results demonstrate significant increases in serum IgG, IgA and 1gM concentrations in both the GEL and EOC subgroups of the BURN and UNBURN groups in comparison to the placebo (NS) group on different postbum days. A significant elevation of IgG was found in the GEL and EOC subgroups of the UNBURN group on days 1 (p=0.026) and 8 (p=0.0l3) respectively. Similar results were observed in the GEL (p=0.001) and EOC (p=0.0l7) subgroups of the BURN group on day 8. There was a significant increase in IgA in the GEL and EOC subgroups for both the BURN and UNBURN groups. In the EOC subgroups, IgA increased significantly on day 15 for the UNBURN group and day 22 for the BURN group (p<0.050 and p=0.029 respectively), whereas in the GEL subgroups, it was found to be higher on day 15 for the UNBURN group and day 22 for the BURN group (p<0.050 and p=0.034 respectively). Conversely, IgA dropped significantly in the GEL subgroup of the BURN group on days 1 and 15 (p=0.038 and p=0.035 respectively). A significant increase in IgM was observed in the EOC subgroups of both the BURN and UNBURN groups and that of the GEL subgroup in the UNBURN group. IgM increased significantly in the EOC subgroup of both the BURN and UNBURN groups, on day 15 (p=0.040) for the BURN group and days 1 and 22 (p=0.010 and p<0.050 respectively) for the UNBURN group, whereas in the GEL subgroup of the UNBURN group it was noted on day 22 only (p=0.025). Blood glucose elevation was only observed in the EOC and GEL subgroups of the BURN group. Significant increases were noted in the EOC on days 1 and 22 (p=0.045 and p=0.0l9 respectively), whereas in the GEL subgroup, the increase was found on day 22 only (p=0.019). Similarly, significant changes in nitric oxide (NO) concentrations were also observed in the EOC and GEL subgroups of the BURN group only. However, there was a significant drop on day 15 for the EOC (p=0.002) and GEL subgroups (p=0.024). Concomitantly, a significant decrease in lipid peroxidation (LPO) was noted in the EOC subgroup of the BURN and UNBURN groups (p=0.006 and p=0.018 respectively), whereas a similar result was only observed in the GEL subgroup of the BURN group (p=0.023) on day 8. Conclusion Results support the contention that EOC enhances serum immunoglobulin concentrations in both normal and bum-inflicted rats. The findings demonstrate that EOC suppressed NO and LPO concentrations in burned rats and that of LPO in normal rats. Therefore, EOC consumption may increase immunological response and prevent free radical-mediated diseases. Further, EOC has shown potential to regulate blood glucose after burn injury by maintaining a stable glucose supply for metabolic needs, which may facilitate wound healing.||Subjects:||Hong Kong Polytechnic University -- Dissertations.
Antioxidants -- Physiological effect.
Free radicals (Chemistry) -- Physiological effect.
Chickens -- Nutritive aspects.
|Pages:||xxi, 127 leaves : ill. (some col.) ; 30 cm.|
|Appears in Collections:||Thesis|
View full-text via https://theses.lib.polyu.edu.hk/handle/200/1589
Citations as of May 15, 2022
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