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Title: Optical mu-printing of cellular-scale microscaffold arrays for 3D cell culture
Authors: Ouyang, X 
Zhang, KY
Wu, JS 
Wong, DSH 
Feng, Q
Bian, LM
Zhang, AP 
Issue Date: 2017
Source: Scientific reports, 21 2017, v. 7, no. , 8880, p. 1-8
Abstract: Guiding cell culture via engineering extracellular microenvironment has attracted tremendous attention due to its appealing potentials in the repair, maintenance, and development of tissues or even whole organs. However, conventional biofabrication technologies are usually less productive in fabricating microscale three-dimensional (3D) constructs because of the strident requirements in processing precision and complexity. Here we present an optical mu-printing technology to rapidly fabricate 3D microscaffold arrays for 3D cell culture and cell-scaffold interaction studies on a single chip. Arrays of 3D cubic microscaffolds with cubical sizes matching the single-cell size were fabricated to facilitate cell spreading on suspended microbeams so as to expose both apical and basal cell membranes. We further showed that the increasing of the cubical size of the microscaffolds led to enhanced spreading of the seeded human mesenchymal stem cells and activation of mechanosensing signaling, thereby promoting osteogenesis. Moreover, we demonstrated that the spatially selective modification of the surfaces of suspended beams with a bioactive coating (gelatin methacrylate) via an in-situ printing process allowed tailorable cell adhesion and spreading on the 3D microscaffolds.
Publisher: Nature Publishing Group
Journal: Scientific reports 
ISSN: 2045-2322
EISSN: 2045-2322
DOI: 10.1038/s41598-017-08598-3
Rights: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2017
The following publication Ouyang, X., Zhang, K., Wu, J. et al. Optical µ-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture. Sci Rep 7, 8880 (2017) is available at https://dx.doi.org/10.1038/s41598-017-08598-3
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