Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/9696
Title: Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway
Authors: Lee, YK
Ng, KM
Lai, WH
Man, C
Lieu, DK
Lau, CP
Tse, HF
Siu, CW
Keywords: Cardiac differentiation
Embryonic stem cells
Ouabain
Issue Date: 2011
Publisher: Shanghai Inst Materia Medica
Source: Acta pharmacologica sinica, 2011, v. 32, no. 1, p. 52-61 How to cite?
Journal: Acta Pharmacologica Sinica 
Abstract: Aim:To investigate the effects of the cardiotonic steroid, ouabain, on cardiac differentiation of murine embyronic stem cells (mESCs).Methods:Cardiac differentiation of murine ESCs was enhanced by standard hanging drop method in the presence of ouabain (20 ?gmol/L) for 7 d. The dissociated ES derived cardiomyocytes were examined by flow cytometry, RT-PCR and confocal calcium imaging.Results:Compared with control, mESCs treated with ouabain (20 ?gmol/L) yielded a significantly higher percentage of cardiomyocytes, and significantly increased expression of a panel of cardiac markers including Nkx 2.5, ?\-MHC, and ?E-MHC. The ?\1 and 2- isoforms Na +/K +-ATPase, on which ouabain acted, were also increased in mESCs during differentiation. Among the three MAPKs involved in the cardiac hypertrophy pathway, ouabain enhanced ERK1/2 activation. Blockage of the Erk1/2 pathway by U0126 (10 ?gmol/L) inhibited cardiac differentiation while ouabain (20 ?gmol/L) rescued the effect. Inte
URI: http://hdl.handle.net/10397/9696
ISSN: 1671-4083
DOI: 10.1038/aps.2010.188
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