Application of oleanolic acid and ursolic acid in preparing medicine for regulating activity of vitamin D3 metabolic enzyme

Abstract

The invention discloses an application of oleanolic acid and ursolic acid in preparing medicine for regulating the activity of vitamin D3 metabolic enzyme, and discovers that natural products oleanolic acid and ursolic acid have the function of regulating the activity of vitamin D3 metabolic enzyme after being mixed according to the mass ratio of 3:1, thereby increasing the level of active vitaminD3(1, 25(OH) 2D3) in vivo, improving calcium balance and enhancing bone density. Natural high molecular zein is used for aligning oleanolic acid and ursolic acid for wrapping on this basis, so that the stability of oleanolic acid and ursolic acid under the external environment condition is improved, the oral bioavailability is increased, meanwhile, the slow release technology of nano particles reduces toxic effects in large-dose oral oleanolic acid and ursolic acid on tissue cells, which can be used in treating or preventing osteoporosis, diabetes, metabolic syndrome, cardiovascular diseases,hyperparathyroidism, renal failure, psoriasis, cancer, and immune diseases due to reduced synthesis of active vitamin D3 and deficiency. 本发明公开了齐墩果酸和熊果酸在制备调节维生素D3代谢酶活性的药物中的应用。本发明发现了天然产物齐墩果酸和熊果酸以质量比3:1混合后，具有调节维生素D3代谢酶活性的作用，从而增加体内活性维生素D3(1,25(OH)2D3)水平，改善钙平衡和增强骨密度。在此基础上，使用天然高分子玉米醇溶蛋白对齐墩果酸和熊果酸进行包裹，提高二者在外界环境条件下的稳定性，增加口服生物利用度，同时纳米颗粒的缓释技术减轻大剂量口服齐墩果酸和熊果酸对组织细胞的毒性作用。可用于治疗或预防活性维生素D3合成减少以及缺乏导致的骨质疏松、糖尿病、代谢综合征、心血管疾病、甲状旁腺亢进、肾衰、牛皮癣、癌症以及免疫疾病。