Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/9086
Title: Emodin induces cytotoxic effect in human breast carcinoma MCF-7 cell through modulating the expression of apoptosis-related genes
Authors: Li, WY
Chan, RYK
Yu, PHF
Chan, SW
Keywords: Anthraquinone
Cell cycle arrest
Growth inhibition
Programmed cell death
Issue Date: 2013
Publisher: Taylor & Francis
Source: Pharmaceutical biology, 2013, v. 51, no. 9, p. 1175-1181 How to cite?
Journal: Pharmaceutical biology 
Abstract: Context: The poor prognostic outcome of breast cancer is largely due to its resistance to cancer therapies. Development of therapeutic agents that can inhibit growth and induce apoptosis in breast cancer cells can help solve the problem. Emodin is an active anthraquinone that has been reported to have diverse biological effects. Objective: In this study, the anticancer effects of emodin on growth inhibition, apoptosis induction and the expression of apoptosis-related genes in MCF-7 cells were investigated. Materials and methods: Growth inhibition induced by emodin was investigated by the MTS assay and the colony formation assay; while emodin-induced apoptosis was determined by the COMET assay and DNA fragmentation detection. Emodin (35 μM)-induced alterations in the expression of apoptotic-related genes were detected by using real-time PCR. Results: Emodin had significant growth inhibitory effects on MCF-7 cells with IC50 = 7.22 μg/ml (~30 μM). It also exerted a concentration-dependant inhibitory effect on the colony-forming ability of MCF-7 cells with IC50 = 7.60 μg/ml (~30 μM). Hallmarks of apoptosis, such as single-strand DNA breakage and DNA fragmentation, were observed in emodin-treated MCF-7 cells. The gene expression of Fas ligand (FASL) was up-regulated (p < 0.01) but those of MCL1, CCND1 and C-MYC were down-regulated (p < 0.05) in emodin-treated MCF-7 cells. Discussion and conclusion: This study indicated that emodin could induce growth inhibition and apoptosis in MCF-7 cells through the modulation of the expression of apoptosis-related genes. The growth inhibitory effects of emodin might involve both the intrinsic and the extrinsic apoptotic pathways and cell cycle arrest.
URI: http://hdl.handle.net/10397/9086
ISSN: 1388-0209
EISSN: 1744-5116
DOI: 10.3109/13880209.2013.782322
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