Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/88439
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dc.contributorDepartment of Applied Biology and Chemical Technologyen_US
dc.creatorZhu, Yen_US
dc.creatorHou, Jen_US
dc.creatorHuang, XHen_US
dc.creatorZhong, DXen_US
dc.creatorLong, Wen_US
dc.creatorLiu, Wen_US
dc.creatorLu, YJen_US
dc.creatorZhang, Ken_US
dc.creatorWong, WLen_US
dc.date.accessioned2020-11-19T03:51:44Z-
dc.date.available2020-11-19T03:51:44Z-
dc.identifier.urihttp://hdl.handle.net/10397/88439-
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2020 Elsevier B.V. All rights reserved.en_US
dc.rights© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.en_US
dc.rightsThe following publication Zhu, Y., Hou, J., Huang, X.-H., Zhong, D.-X., Long, W., Liu, W., Lu, Y.-J., Zhang, K., & Wong, W.-L. (2020). Structural modification of nonspecific thiazole orange for ligand-DNA interaction study: Understanding the ligand recognition selectivity towards G4-DNA over duplex-DNA. Journal of Luminescence, 226, 117488 is available at https://dx.doi.org/10.1016/j.jlumin.2020.117488.en_US
dc.subjectFluorescent binding liganden_US
dc.subjectG-quadruplex DNA recognitionen_US
dc.subjectLigand-DNA interactionen_US
dc.subjectMolecular dockingen_US
dc.titleStructural modification of nonspecific thiazole orange for ligand-DNA interaction study : understanding the ligand recognition selectivity towards G4-DNA over duplex-DNAen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage1en_US
dc.identifier.epage9en_US
dc.identifier.volume226en_US
dc.identifier.doi10.1016/j.jlumin.2020.117488en_US
dcterms.abstractThe study of ligand interactions with nucleic acid structures such as G-quadruplexes versus double-stranded DNA is important because these interactions are fundamental for many intracellular processes. In the investigation of ligand-DNA binding process, achieving high fluorescent signal discrimination with strong binding affinity is challenging. To develop binding ligands with excellent recognition ability towards G4-DNA over duplex-DNA, the design of appropriate molecular scaffolds that are able to match with the G4-DNA binding pocket (G-quartet) is crucial. In the present study, the new fluorescent DNA binding ligands were designed and synthesized through the integration of a small and conjugated substituent group at the 2-position of the 1-methylquinolinium moiety of the nonspecific thiazole orange scaffold. The ligands were investigated in fluorescence binding assays and showed different interaction properties and significant fluorescent recognition selectivity towards G4-DNA over duplex-DNA in vitro. Molecular docking study of the ligands in complex with telo21 G4-DNA and ds26 duplex DNA also revealed different binding modes. In addition, the cytotoxicity of the fluorescent DNA binding ligands was evaluated in MTT assays against two selected cancer cell lines (human prostate cancer cell (PC3) and human hepatoma cell (hepG2)). The IC50 was found in the range of 6.3–12.5 μM, indicating a relatively high cytotoxicity of the ligands towards the cancer cells examined.en_US
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationJournal of luminescence, Oct. 2020, v. 226, 117488, p. 1-9en_US
dcterms.isPartOfJournal of luminescenceen_US
dcterms.issued2020-10-
dc.identifier.scopus2-s2.0-85088629483-
dc.identifier.eissn0022-2313en_US
dc.identifier.artn117488en_US
dc.description.validate202011 bcrcen_US
dc.description.oaAccepted Manuscripten_US
dc.identifier.FolderNumbera0505-n01-
dc.description.pubStatusPublisheden_US
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