Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/8556
DC FieldValueLanguage
dc.contributorDepartment of Health Technology and Informatics-
dc.creatorTam, BT-
dc.creatorPei, XM-
dc.creatorYu, AP-
dc.creatorSin, TK-
dc.creatorLeung, KK-
dc.creatorAu, KK-
dc.creatorChong, JT-
dc.creatorYung, BY-
dc.creatorYip, SP-
dc.creatorChan, LW-
dc.creatorWong, CS-
dc.creatorSiu, PM-
dc.date.accessioned2015-10-13T08:25:56Z-
dc.date.available2015-10-13T08:25:56Z-
dc.identifier.issn1748-1708-
dc.identifier.urihttp://hdl.handle.net/10397/8556-
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.subjectAutophagyen_US
dc.subjectChronic exerciseen_US
dc.subjectFibre shiftingen_US
dc.subjectLC3en_US
dc.subjectSkeletal muscleen_US
dc.titleAutophagic adaptation is associated with exercise-induced fibre-type shifting in skeletal muscleen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage221-
dc.identifier.epage236-
dc.identifier.volume214-
dc.identifier.issue2-
dc.identifier.doi10.1111/apha.12503-
dcterms.abstractAim: Acute exercise is known to activate autophagy in skeletal muscle. However, little is known about how basal autophagy in skeletal muscle adapts to chronic exercise. In the current study we aim to, firstly, examine whether long-term habitual exercise alters the basal autophagic signalling in plantaris muscle and, secondly, examine the association between autophagy and fibre-type shifting. Methods: Adult female Sprague-Dawley rats aged 2 months were randomly assigned to control and exercise groups. Animals in exercise group were kept in cages equipped with free access running wheels to perform habitual exercise for 5 months. Animals in the control group were caged in the absence of running wheels. Animals were sacrificed after the 5-month experimental period. Plantaris muscle tissues were harvested for analysis. Results: We showed that long-term habitual exercise enhanced basal autophagy, but without altering expressions of autophagy proteins in plantaris muscle. Interestingly, sirtuin protein, a possible regulator of autophagy, was upregulated in plantaris muscle. Furthermore, we suspected that different types of muscle fibre adapted to chronic exercise in different ways. Long-term habitual exercise resulted in fibre-type shifting from type IIX to IIA in both gastrocnemius muscle and plantaris muscle. Intriguingly, our analysis demonstrated that LC3-II protein abundance is positively correlated with the proportion of type IIA fibre whereas it was negatively correlated with the proportion of type IIX fibre in plantaris muscle. PGC-1£\ protein abundance was positively associated with the proportion of type IIA fibre and LC3-II in plantaris muscle. Conclusion: These results suggest that basal autophagy is enhanced in plantaris muscle after long-term habitual exercise and associated with fibre-type shifting.-
dcterms.bibliographicCitationActa physiologica, 2015, v. 214, no. 2, p. 221-236-
dcterms.isPartOfActa physiologica-
dcterms.issued2015-
dc.identifier.scopus2-s2.0-84929274635-
dc.identifier.eissn1748-1716-
dc.identifier.rosgroupid2014001158-
dc.description.ros2014-2015 > Academic research: refereed > Publication in refereed journal-
Appears in Collections:Journal/Magazine Article
Access
View full-text via PolyU eLinks SFX Query
Show simple item record

SCOPUSTM   
Citations

24
Last Week
1
Last month
1
Citations as of Aug 29, 2020

WEB OF SCIENCETM
Citations

25
Last Week
0
Last month
1
Citations as of Oct 22, 2020

Page view(s)

168
Last Week
0
Last month
Citations as of Oct 27, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.