Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/8455
Title: The human HMGB1 promoter is modulated by a silencer and an enhancer-containing intron
Authors: Lum, HK
Lee, KL
Issue Date: 2001
Source: Biochimica et biophysica acta. Gene structure and expression, 2001, v. 1520, no. 1, p. 79-84
Abstract: The highly conserved, ubiquitous high mobility group protein HMGB1 (formerly named as HMG1) is an architectural transcription factor encoded by a single functional gene in human. HMGB1 is expressed in almost all cell or tissue types studied. In general, it is expressed at a basal level in most cells but at a slightly elevated level of 2-3-fold in actively proliferating tissues or estrogen stimulated breast cancer cells. To understand the regulatory mechanism controlling expression of the human HMGB1 gene, we cloned and analyzed the upstream region as well as the first intron of this gene. We found that transcription of the human HMGB1 gene in the breast cancer MCF-7 cells starts at one major site 57 nucleotides upstream from the first exon-intron boundary. Expression of the human HMGB1 gene is under the control of a very strong TATA-less promoter, which has an activity more than 18-fold that of the SV40 promoter. Immediately upstream, a silencer element is present. This silencer can repress the activity of the HMGB1 promoter down to just one-sixth. The first intron of the human HMGB1 gene contains enhancer elements, which can increase the human HMGB1 promoter activity by 2-3-fold. We postulate that the human HMGB1 gene is capable of being expressed at a very high level. The basal level of expression observed in most cells is probably a result of the strong promoter being held in check by the silencer. The 2-3-fold increase in HMGB1 expression observed in proliferating cells or breast cancer cells stimulated by estrogen may probably result from the action of the enhancer elements in intron 1.
Keywords: High mobility group 1 gene
High mobility group protein B1
Intron enhancer
Promoter
Silencer
Publisher: Elsevier
Journal: Biochimica et biophysica acta. Gene structure and expression 
ISSN: 0167-4781
DOI: 10.1016/S0167-4781(01)00243-3
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

24
Last Week
0
Last month
0
Citations as of Sep 2, 2020

WEB OF SCIENCETM
Citations

23
Last Week
0
Last month
0
Citations as of Sep 17, 2020

Page view(s)

141
Last Week
0
Last month
Citations as of Sep 13, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.