Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/81552
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dc.contributor.authorLam, Cen_US
dc.contributor.authorLi, KKen_US
dc.contributor.authorDo, CWen_US
dc.contributor.authorChan, Hen_US
dc.contributor.authorTo, CHen_US
dc.contributor.authorKwong, JMKen_US
dc.date.accessioned2019-10-28T05:46:01Z-
dc.date.available2019-10-28T05:46:01Z-
dc.date.issued2019-
dc.identifier.citationMolecular medicine reports, 2019, v. 20, no. 3, p. 2734-2742en_US
dc.identifier.issn1791-2997-
dc.identifier.urihttp://hdl.handle.net/10397/81552-
dc.description.abstractTo examine the difference between primary and secondary retinal ganglion cell (RGC) degeneration, the protein expression at four regions of retina including superior, temporal, inferior and nasal quadrant in a rat model of partial optic nerve transection (pON T) using 2-D Fluorescence Difference Gel Electrophoresis (DI GE) were investigated. Unilateral pON T was performed on the temporal side of optic nerves of adult Wistar rats to separate primary and secondary RGC loss. Topographical quantification of RGCs labeled by Rbpms antibody and analysis of axonal injury by grading of optic nerve damage at 1 week (n=8) and 8 weeks (n=15) after pON T demonstrated early RGC loss at temporal region, which is considered as primary RGC degeneration and progressing RGC loss at nasal region, which is considered as secondary RGC degeneration. Early protein expression in each retinal quadrant (n=4) at 2 weeks after pON T was compared with the corresponding quadrant in the contralateral control eye by DI GE. For all comparisons, 24 differentially expressed proteins (>1.2-fold; P<0.05; =3 non-duplicated peptide matches) were identified by mass spectrometry (MS). Interestingly, in the nasal retina, serum albumin and members of crystallin family, including αA, αB, βA2, βA3, βB2 and γS indicating stress response were upregulated. By contrast, only αB and βA2 crystallin proteins were altered in temporal quadrant. In the superior and inferior quadrants, βB2 crystallin, keratin type I, S-arrestin and lamin-B1 were upregulated, while heat shock cognate 71 kDa protein and heterogeneous nuclear ribonucleoproteins A2/B1 were downregulated. In summary, the use of DI GE followed by MS is useful to detect early regional protein regulation in the retina after localized optic nerve injury.en_US
dc.description.sponsorshipSchool of Optometryen_US
dc.language.isoenen_US
dc.publisherSpandidos Publicationsen_US
dc.relation.ispartofMolecular medicine reportsen_US
dc.rights© Lam et al. This is an open access article distributed under the terms of Creative Commons Attribution License.en_US
dc.rightsThe following publication Lam, C., Li, K.K., Do, C.W., Chan, H., To, C.H., & Kwong, J.M. (2019). Quantitative profiling of regional protein expression in rat retina after partial optic nerve transection using fluorescence difference two‑dimensional gel electrophoresis. Molecular Medicine Reports, 20, 2734-2742 is available at https://doi.org/10.3892/mmr.2019.10525en_US
dc.subjectNeurodegenerationen_US
dc.subjectOptic nerveen_US
dc.subjectProteinen_US
dc.subjectProteomicsen_US
dc.subjectRetinaen_US
dc.subjectRetinal ganglion cellen_US
dc.titleQuantitative profiling of regional protein expression in rat retina after partial optic nerve transection using fluorescence difference two-dimensional gel electrophoresisen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage2734-
dc.identifier.epage2742-
dc.identifier.volume20-
dc.identifier.issue3-
dc.identifier.doi10.3892/mmr.2019.10525-
dc.identifier.scopus2-s2.0-85070555823-
dc.identifier.eissn1791-3004-
dc.description.validate201910 bcma-
dc.description.oapublished_final-
Appears in Collections:Journal/Magazine Article
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