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dc.contributor.advisorLeung, Thomas (ABCT)en_US
dc.contributor.advisorTsang, Edman (ABCT)en_US
dc.contributor.authorChan, Zoeen_US
dc.descriptionxvi, 170 pages : color illustrationsen_US
dc.descriptionPolyU Library Call No.: [THS] LG51 .H577P ABCT 2019 Chanen_US
dc.description.abstractTuberculosis has been one of the deadliest disease in history. Although it became curable since the discovery of antibiotics, drug resistance was soon developed and the needs in new antituberculosis treatment escalated. In the past, our group has developed an effective β-lactamase-based fluorescent biosensor for β-lactam antibiotics detection. BlaC, a β-lactamase responsible for the β-lactam antibiotic resistance in Mycobacterium tuberculosis, was engineered to be a sensitive and efficient biosensor for screening potential inhibitors to be used in combination with currently available β-lactam antibiotics. Thr-216 was mutated to Cys by site-directed mutagenesis and was named T216C. Various fluorophores were labelled on the mutated residue and the prospective fluorescent sensor enzyme was screened. The functionality of the most promising fluorophore-labelled enzyme T216Cf against antibiotics and inhibitors was investigated with fluorescence spectrometry. Immediate surges in fluorescence intensity of T216Cf upon penicillins addition and positive signals induced by carbapenems and inhibitors were observed, but the response to cephalosporins was insignificant. The relationship between enzyme-inhibitor complex formation and fluorescence intensity change was also revealed by the combination of ESI-MS and fluorescence spectrometry. The kinetic parameters were measured and calculated to examine the impact of mutagenesis and labelling. A second mutation to Trp was introduced at Ile-105 and Thr-237 in an attempt to enhance the biosensor sensitivity. T216Cf/I105W showed a major improvement in sensitivity, as much as from +35% to +83% upon inhibitor addition, although further optimisation can be done on the biosensor efficiency. Molecular dynamics simulations were conducted to understand the mechanism behind the fluorescence signals, which is transferable to similar β-lactamase-based biosensor. The change in residues in proximity to the fluorescein, solvent exposure, and interaction with quenchers such as tryptophan all contribute to the fluorescence signals. The promising results suggested that the new T216Cf has great potential to be refined as a powerful biosensor for drug screening.en_US
dc.description.sponsorshipDepartment of Applied Biology and Chemical Technologyen_US
dc.publisherThe Hong Kong Polytechnic Universityen_US
dc.rightsAll rights reserved.en_US
dc.subjectAntitubercular agentsen_US
dc.titleDevelopment of β-lactamase-based biosensor for anti-tuberculosis drug screeningen_US
dc.description.degreePh.D., Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, 2019en_US
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