Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/81106
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dc.contributorDepartment of Rehabilitation Sciences-
dc.creatorChow, DH-
dc.creatorZheng, LZ-
dc.creatorTian, L-
dc.creatorHo, KS-
dc.creatorQin, L-
dc.creatorGuo, X-
dc.date.accessioned2019-07-29T03:17:59Z-
dc.date.available2019-07-29T03:17:59Z-
dc.identifier.issn2214-031X-
dc.identifier.urihttp://hdl.handle.net/10397/81106-
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights©2018 The Authors. Published by Elsevier (Singapore) Pte Ltd on behalf of Chinese Speaking Orthopaedic Society. This is an openaccess article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)en_US
dc.rightsThe following publication Chow, D. H., Zheng, L. Z., Tian, L., Ho, K. S., Qin, L., … & Guo, X. (2019). Application of ultrasound accelerates the decalcification process of bone matrix without affecting histological and immunohistochemical analysis. Journal of orthopaedic translation, 17, 112-120 is available at https://dx.doi.org/10.1016/j.jot.2018.08.001en_US
dc.subjectBone histologyen_US
dc.subjectBone histomorphometryen_US
dc.subjectDecalcificationen_US
dc.subjectImmuno histochemistryen_US
dc.subjectUltrasounden_US
dc.titleApplication of ultrasound accelerates the decalcification process of bone matrix without affecting histological and immunohistochemical analysisen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage112-
dc.identifier.epage120-
dc.identifier.volume17-
dc.identifier.doi10.1016/j.jot.2018.08.001-
dcterms.abstractBackground/Objectives: Decalcification of bone specimens is necessary for routine paraffin embedding and sectioning. Ethylenediaminetetraacetic acid (EDTA), a chelating agent for decalcification, maintains bone tissue integrity and histological features but requires long decalcification period, especially for cortical bone with dense mineral matrix. We hypothesised that the application of a newly commercially available ultrasound (US) decalcifier would accelerate decalcification of thick cortical bone specimen in EDTA efficiently and that the working temperature at 30-45 degrees C would not affect histological and immunohistochemical analysis. Comparison was made with traditional decalcification method with regards to quality of tissue morphology and antigenicity.-
dcterms.abstractMethods: A fresh human cadaveric femoral shaft was sectioned into 5-mm-thick transverse sections. After fixation, the bone slices were divided into two groups: Ultrasound decalcification group (US DeCal), in which bone sections (n = 3) were placed in a US decalcifier (50 W at a frequency of 40kHz) with EDTA solution, and normal decalcification group (Normal DeCal), in which bone sections (n = 3) were decalcified in EDTA without US. The mineral content of the bone sections was measured with micro-computed tomography and dual-energy X-ray absorptiometry at different time points. Rate of calcium extraction was quantified by measuring the calcium concentration in EDTA solution using inductively coupled plasma optical emission spectrometry. After decalcification, the paraffin sections of the decalcified bone were stained with haematoxylin and eosin or immunohistochemical staining of sclerostin.-
dcterms.abstractResults: Samples in US DeCal contained 2.9 +/- 2.8% of the mineral content at Day 6 and were completely decalcified at Day 8. However, sections in Normal DeCal retained 36.3 +/- 5.1% and 24.3 +/- 4.8% at Day 6 and Day 8, respectively, and took six times longer to complete decalcification. The concentration of calcium in the EDTA solution of the US DeCal group was 70% higher than that of the Normal DeCal group (p < 0.05) in Day 1 and 2. No staining difference was observed in histological sections between the two groups.-
dcterms.abstractConclusion: The application of US decalcification significantly shortened the decalcification time in EDTA without causing histological artefacts. The translational potential of this article: This article shows that the application of ultrasound in sample decalcification would shorten the duration that decalcification required. This would accelerate the sample processing for routine bone histology in both basic and clinical research and assessments for diagnostic purposes.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationJournal of orthopaedic translation, Apr. 2019, v. 17, p. 112-120-
dcterms.isPartOfJournal of orthopaedic translation-
dcterms.issued2019-
dc.identifier.isiWOS:000470125300012-
dc.identifier.pmid31194084-
dc.description.validate201907 bcrc-
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_Scopus/WOSen_US
dc.description.pubStatusPublisheden_US
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