Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/8030
Title: Molecular cloning and the biochemical characterization of two novel phytases from B. subtilis 168 and B. licheniformis
Authors: Tye, A
Siu, F
Leung, T 
Lim, B
Issue Date: 2002
Source: Applied microbiology and biotechnology, 2002, v. 59, no. 2-3, p. 190-197 How to cite?
Journal: Applied Microbiology and Biotechnology 
Abstract: A novel phytase gene (phyL) was cloned from Bacillus licheniformis by multiple steps of degenerate and inverse PCR. The coding region of the phyL gene was 1,146 bp in size and a promoter region of approximately 300 bp was identified at the upstream sequence. This gene, together with a phytase gene (168phyA) identified in the B. subtilis strain 168 genome by a homology search, was cloned and over-expressed in B. subtilis using a φ105MU331 prophage vector system. Up to 35 units of phytase/ml were secreted into the culture media; and mature enzymes of around 44-47 kDa were purified for characterization. Both phytases exhibited broad temperature and pH optima and showed high thermostability. Of the two, the phytase encoded by phyL exhibited higher thermostability, even at a lower calcium concentration, as it was able to recover 80% of its original activity after denaturation at 95°C for 10 min. With their neutral pH optima and good temperature stabilities, these Bacillus phytases are good candidates for animal feed applications and transgenic studies.
URI: http://hdl.handle.net/10397/8030
ISSN: 0175-7598
DOI: 10.1007/s00253-002-1033-5
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

108
Last Week
0
Last month
1
Citations as of Jun 24, 2017

WEB OF SCIENCETM
Citations

90
Last Week
0
Last month
0
Citations as of Jun 22, 2017

Page view(s)

35
Last Week
0
Last month
Checked on Jun 25, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.