Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/79290
Title: Recombination of plasmids in a carbapenem-resistant NDM-5-producing clinical Escherichia coli isolate
Authors: Xie, MM 
Li, RC 
Liu, ZH
Chan, EWC 
Chen, S 
Issue Date: 2018
Publisher: Oxford University Press
Source: Journal of antimicrobial chemotherapy, May 2018, v. 73, no. 5, p. 1230-1234 How to cite?
Journal: Journal of antimicrobial chemotherapy 
Abstract: Objectives: To investigate the genetic features of five plasmids recovered from an NDM-5-producing clinical Escherichia coli strain, BJ114, and to characterize the plasmid recombination event that occurred during the conjugation process.
Methods: The genetic profiles of the five plasmids were determined by PCR, conjugation, S1-PFGE, Southern hybridization and WGS analysis. Plasmid sequences were analysed with various bioinformatic tools.
Results: Complete sequences of five plasmids were obtained. Two small plasmids, pBJ114-141 and pBJ114-46, were speculated to have recombined into a large fusion plasmid, pBJ114T-190. When conjugated to other E. coil strains, some of the fusion plasmids were able to be resolved into the original two single plasmids. A non-conjugative plasmid, pBJ114-96, exhibited a high degree of sequence identity with the phage P7-like plasmid as well as an mcr-1-bearing plasmid. Another plasmid, pBJ114-78, was found to contain multidrug resistance genes and various mobile elements.
Conclusions: The fusion plasmid recoverable from the transconjugant was found to be generated as a result of a recombination event that occurred upon interaction between a blan(NDm)(-5)-carrying plasmid and another plasmid present in the parental strain. Such recombination events presumably play a potential role in the dissemination of the bla(NDM) genes among different plasmids and pathogenic bacterial strains.
URI: http://hdl.handle.net/10397/79290
ISSN: 0305-7453
EISSN: 1460-2091
DOI: 10.1093/jac/dkx540
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