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Title: Chemical characterization of essential oil in Rhizoma asarum from different sources using GC-MS with resolution improved by data processing techniques
Authors: Gong, F
Wang, B
Chau, F
Keywords: Chemometrics
Data processing
Essential oil
Rhizoma asarum
Issue Date: 2006
Source: Flavour and fragrance journal, 2006, v. 21, no. 3, p. 549-555 How to cite?
Journal: Flavour and Fragrance Journal 
Abstract: Rhizoma asarum has been widely used both in phytotherapy and in the food industry because of its pharmacological activities and odoriferous properties. However, besides desirable pharmacological effects, asarum oil is also found to exhibit toxic effects. In this study, gas chromatography-mass spectrometry (GC-MS) with resolution improved by data processing techniques in chemometrics was investigated as a method to chemically characterize essential oils of raw materials of Rhizoma asarum from Liaoning, Gansu and Hunan in China. With the help of chemometric approaches on two-dimensional data from GC-MS, the separation ability of GC was mathematically improved and many more chemical components could be separated and identified since overlapping peak clusters could be resolved into pure chromatograms and related mass spectra for each of the components involved. Qualitative and quantitative determination was conducted with the pure chromatograms and mass spectra obtained by use of similarity searches in the MS databases and the overall volume integration technique, respectively. Moreover, the information on retention times was also used for the identification. The results showed that 96, 94 and 93 chemical components were separated; 69, 67 and 66 of them were tentatively identified, which accounted for about 97.68, 97.51 and 97.63% of the total relative content of the essential oils of Rhizoma asarum from Liaoning, Gansu and Hunan, respectively. In comparison with the determined constituents in essential oils of Rhizoma asarum from three producing sources, most of them matched each other.
ISSN: 0882-5734
DOI: 10.1002/ffj.1664
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