Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/77730
Title: Genetic basis of chromosomally-encoded mcr-1 gene
Authors: Li, R 
Yu, H
Xie, M 
Chen, K 
Dong, N 
Lin, D 
Chan, EWC 
Chen, S 
Keywords: Chromosome
Circular intermediate
Escherichia coli
Mcr-1
Tn6330
Transmission
Issue Date: 2018
Publisher: Elsevier
Source: International journal of antimicrobial agents, 2018, v. 51, no. 4, p. 578-585 How to cite?
Journal: International journal of antimicrobial agents 
Abstract: Compared with plasmid-borne mcr-1, the occurrence of chromosomally-encoded mcr-1 is rare although it has been reported in several cases. This study aimed to investigate the genetic features of chromosomally-encoded mcr-1 among Escherichia coli strains as well as the potential genetic basis governing mobilisation of mcr-1 in bacterial chromosomes. The genome sequences of 16 E. coli strains containing a chromosomal mcr-1 gene were obtained and analysed. Phylogenetic and whole-genome sequencing (WGS) analysis demonstrated that mcr-1 was associated with four major types of genetic arrangements, namely ISApl1–mcr1–orf, Tn6330, complex Tn6330 and ΔTn6330 in chromosomes of genetically unrelated E. coli strains. The mcr-1-carrying mobile elements were shown to insert into the AT-rich region, which was also the case for ISApl1. Analysis of complete E. coli genome sequences showed that there were multiple copies of ISApl1 present in E. coli chromosomes that also carried mcr-1, whilst all mcr-1-negative chromosomes were absent of any copy of ISApl1, suggesting the strong association of ISApl1 and mcr-1. Insertion of ISApl1 into E. coli chromosomes may be a prerequisite for the insertion of mcr-1-carrying mobile elements. Insertion of mcr-1 into E. coli chromosomes would enable it to become intrinsically resistant, which is expected to become more prevalent. Policy on the prudent use of colistin both in veterinary and clinical settings should be imposed globally to further prevent dissemination of mcr-1 in E. coli and other bacterial pathogens.
URI: http://hdl.handle.net/10397/77730
ISSN: 0924-8579
EISSN: 1872-7913
DOI: 10.1016/j.ijantimicag.2017.11.015
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

1
Citations as of Sep 11, 2018

WEB OF SCIENCETM
Citations

1
Citations as of Sep 18, 2018

Page view(s)

2
Citations as of Sep 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.