Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/76811
Title: Method and apparatus for detecting specific DNA sequences
Other Titles: 检测特定DNA序列的方法和装置
Authors: Or, SW 
Lo, CLS 
Issue Date: 14-May-2014
Source: 中国专利 ZL 201110155007.7 How to cite?
Abstract: An apparatus for detecting the presence of a microorganism in a sample includes a housing that includes a base fixed with a first DNA primer having a nucleotide sequence that is complementary to a DNA sequence of the microorganism of interest, a fibrinogen-splitting agent that is bound with a second DNA primer having a nucleotide sequence that is also complementary to a DNA sequence of the microorganism of interest, a rinsing unit configured to rinse the housing; and a fibrinogen adding unit configured to add fibrinogen to the housing so that the fibrinogen chemically reacts with the fibrinogen-splitting agent to produce a viscous substance, an ultrasonic emitter configured to emit ultrasonic signal to the housing, and an ultrasonic receiver configured to receive ultrasonic signal from the housing and transmit the received ultrasonic signal to an ultrasonic analyzer, wherein the ultrasonic analyzer determines whether the microorganism of interest exists.
检测样品中微生物的存在的装置包括壳体,所述壳体包括与第一DNA引物固定的基底,所述第一DNA引物具有与感兴趣的微生物的特定DNA序列互补的核苷酸序列;与第二DNA引物结合的纤维蛋白原分裂剂,所述第二DNA引物具有同样与目的微生物的DNA序列互补的核苷酸序列;用于清洗所述壳体的清洗单元;用于向所述壳体添加纤维蛋白原的纤维蛋白原添加单元以便纤维蛋白原与纤维蛋白原分裂剂发生化学反应产生粘性物质;用于向所述壳体发射超声信号的超声发射器;以及用于接收来自于所述壳体的超声信号并将接收的超声信号传输至超声分析器的超声接收器,其中所述超声分析器基于接收的超声信号确定是否存在感兴趣的微生物。
URI: http://hdl.handle.net/10397/76811
Rights: Assignee: The Hong Kong Polytechnic University
Appears in Collections:Patent

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