Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/76690
Title: Sichuan pepper attenuates H2O2-induced apoptosis via antioxidant activity and up-regulating heme oxygenase-1 gene expression in primary rat hepatocytes
Authors: Zhang, H 
Zhou, X
Wong, MHY 
Man, KY 
Pin, WK 
Yeung, JHK
Kwan, YW
Leung, GPH
Hoi, PM
Lee, SMY
Chan, CO
Mok, DKW 
Yu, PHF
Chan, SW
Keywords: Liver
Reactive oxygen species
Rutaceae
Zanthoxylum bungeanum Maxim
Issue Date: 2017
Publisher: John Wiley & Sons
Source: Journal of food biochemistry, 2017, p. 2 How to cite?
Journal: Journal of food biochemistry 
Abstract: Sichuan pepper, a well-known traditional Chinese spice, is popularly used in Chinese cuisine. In this study, the antioxidant activities of Sichuan pepper water extract (SP) were evaluated both chemically [in 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay] and biologically [in 2,2′-azobis-(2-amidinopropane) (AAPH)-induced haemolysis assay]. The protective effect of SP against H2O2-induced cytotoxicity in primary Sprague Dawley rat hepatocytes was investigated by using cell viability assay, nuclear staining, intracellular reactive oxygen species (ROS) measurement and flow cytometry. It was found to possess high total phenolic content as assessed by the Folin-Ciocalteu method. The antiapoptotic effect of SP may be mediated, in part, by significantly inhibiting intracellular ROS generation and depletion of the mitochondrial membrane potential. SP was also found to enhance the cellular antioxidant defense system through up-regulating heme oxygenase-1 expression. SP is an effective antioxidant that can be consumed regularly as a functional food for the prevention of oxidative stress-related liver diseases. Practical applications: The present study demonstrated that Sichuan pepper water extract (SP) could enhance the cellular antioxidant ability in hepatocytes. SP is an effective antioxidant that can be consumed regularly as a functional food for the prevention of oxidative stress-related liver diseases.
URI: http://hdl.handle.net/10397/76690
ISSN: 0145-8884
EISSN: 1745-4514
DOI: 10.1111/jfbc.12403
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