Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/76420
Title: Optimizing a protein's expression in an efficient way
Authors: Li, Z 
Li, Y
Dai, FY
Keywords: E. coli
Prokaryotic expression
Growth conditions
Optimization
DoE
Issue Date: 2017
Publisher: Research Journal Biotechnology
Source: Research journal of biotechnology, 2017, v. 12, no. 3, p. 15-21 How to cite?
Journal: Research journal of biotechnology 
Abstract: Prokaryotic expression is a common way for heterologous expression of proteins. For achieving high-level expression in E. coli, optimization is essential followed by the recombinant protein. The conditions optional for optimization contain host strain, expression vector design, gene dosage, codon usage, mRNA level and growth conditions/media. A series of factors tightly related to growth conditions involving incubate temperature, OD600 value, IPTG concentration, antibiotic concentration and time of induction have known effect on a protein's expression. Generally, 'One factor at a time' method is used to optimize a protein's expression based on these factors; however, this efficiency is low and the connections between factors are thoroughly ignored. Here, design of experiment (DoE) is introduced for optimization of protein expression in this study revealing an interaction between OD600 and incubate time which can significantly influence the expression level of our recombinant protein Bmattacin2 in addition to each separate one. The result of optimization verifies that DoE is an efficient way for optimizing a protein's expression since it can significantly improve protein expression level compared with conventional 'One factor at a time' method.
URI: http://hdl.handle.net/10397/76420
ISSN: 2278-4535
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