Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/68567
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dc.contributor.authorLi, RCen_US
dc.contributor.authorXie, MMen_US
dc.contributor.authorLv, JZen_US
dc.contributor.authorChan, EWCen_US
dc.contributor.authorChen, Sen_US
dc.date.accessioned2017-08-29T02:19:23Z-
dc.date.available2017-08-29T02:19:23Z-
dc.date.issued2017-
dc.identifier.citationJournal of antimicrobial chemotherapy, 2017, v. 72, no. 3, p. 696-699en_US
dc.identifier.issn0305-7453en_US
dc.identifier.urihttp://hdl.handle.net/10397/68567-
dc.description.abstractObjectives: To investigate the genetic features of three plasmids recovered from an MCR-1 and ESBL-producing Escherichia coli strain, HYEC7, and characterize the transmission mechanism of mcr-1.en_US
dc.description.abstractMethods: The genetic profiles of three plasmids were determined by PCR, S1-PFGE, Southern hybridization and WGS analysis. The ability of the mcr-1-bearing plasmid to undergo conjugation was also assessed. The mcr-1bearing transposon Tn6330 was characterized by PCR and DNA sequencing.en_US
dc.description.abstractResults: Complete sequences of three plasmids were obtained. A non-conjugative phage P7-like plasmid, pHYEC7-mcr1, was found to harbour the mcr-1-bearing transposon Tn6330, which could be excised from the plasmid by generating a circular intermediate harbouring mcr-1 and the ISApl1 element. The insertion of the circular intermediate into another plasmid, pHYEC7-IncHI2, could form pHNSHP45-2, the original IncHI2-type mcr1-carrying plasmid that was reported. The third plasmid, pHYEC7-110, harboured two replicons, IncX1 and IncFIB, and comprised multiple antimicrobial resistance mobile elements, some of which were shared by pHYEC7-IncHI2.en_US
dc.description.abstractConclusions: The Tn6330 element located in the phage-like plasmid pHYEC7-mcr1 could be excised from the plasmid and formed a circular intermediate that could be integrated into plasmids containing the ISApl1 element. This phenomenon indicated that Tn6330 is a key element responsible for widespread dissemination of mcr-1 among various types of plasmids and bacterial chromosomes. The dissemination rate of such an element may be further enhanced upon translocation into phage-like vectors, which may also be transmitted via transduction events.en_US
dc.description.sponsorshipDepartment of Applied Biology and Chemical Technologyen_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofJournal of antimicrobial chemotherapyen_US
dc.titleComplete genetic analysis of plasmids carrying mcr-1 and other resistance genes in an Escherichia coli isolate of animal originen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.spage696en_US
dc.identifier.epage699en_US
dc.identifier.volume72en_US
dc.identifier.issue3en_US
dc.identifier.doi10.1093/jac/dkw509en_US
dc.identifier.isiWOS:000398038800006-
dc.identifier.pmid27999050-
dc.source.typeArticleen
dc.identifier.eissn1460-2091en_US
item.fulltextFull Text (via PolyU elinks)-
crisitem.author.deptDepartment of Applied Biology and Chemical Technology-
crisitem.author.facultyFaculty of Applied Science and Textiles-
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