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Title: Complete genetic analysis of plasmids carrying mcr-1 and other resistance genes in an Escherichia coli isolate of animal origin
Authors: Li, RC 
Xie, MM
Lv, JZ
Chan, EWC 
Chen, S 
Issue Date: 2017
Publisher: Oxford University Press
Source: Journal of antimicrobial chemotherapy, 2017, v. 72, no. 3, p. 696-699 How to cite?
Journal: Journal of antimicrobial chemotherapy 
Abstract: Objectives: To investigate the genetic features of three plasmids recovered from an MCR-1 and ESBL-producing Escherichia coli strain, HYEC7, and characterize the transmission mechanism of mcr-1.
Methods: The genetic profiles of three plasmids were determined by PCR, S1-PFGE, Southern hybridization and WGS analysis. The ability of the mcr-1-bearing plasmid to undergo conjugation was also assessed. The mcr-1bearing transposon Tn6330 was characterized by PCR and DNA sequencing.
Results: Complete sequences of three plasmids were obtained. A non-conjugative phage P7-like plasmid, pHYEC7-mcr1, was found to harbour the mcr-1-bearing transposon Tn6330, which could be excised from the plasmid by generating a circular intermediate harbouring mcr-1 and the ISApl1 element. The insertion of the circular intermediate into another plasmid, pHYEC7-IncHI2, could form pHNSHP45-2, the original IncHI2-type mcr1-carrying plasmid that was reported. The third plasmid, pHYEC7-110, harboured two replicons, IncX1 and IncFIB, and comprised multiple antimicrobial resistance mobile elements, some of which were shared by pHYEC7-IncHI2.
Conclusions: The Tn6330 element located in the phage-like plasmid pHYEC7-mcr1 could be excised from the plasmid and formed a circular intermediate that could be integrated into plasmids containing the ISApl1 element. This phenomenon indicated that Tn6330 is a key element responsible for widespread dissemination of mcr-1 among various types of plasmids and bacterial chromosomes. The dissemination rate of such an element may be further enhanced upon translocation into phage-like vectors, which may also be transmitted via transduction events.
ISSN: 0305-7453
EISSN: 1460-2091
DOI: 10.1093/jac/dkw509
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