Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/66475
Title: Effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs
Authors: Wang, Z
Qing, Q
Chen, X
Liu, CJ
Luo, JC
Hu, JL 
Qin, TW
Keywords: Tenocyte
Scaffold
Surface morphology
Vitreous cryopreservation
Adhesion
Survival rate
Issue Date: 2016
Publisher: North-Holland
Source: Applied surface science, 1 Dec. 2016, v. 388, part A, p. 223-227 How to cite?
Journal: Applied surface science 
Abstract: The purpose of this study was to investigate the effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs. Tenocytes were obtained from tail tendons of rats. Polydimethylsiloxane (PDMS) was used to fabricate three types of scaffolds with varying surface morphological characteristics, i.e., smooth, micro-grooved, and porous surfaces, respectively. The tenocytes were seeded on the surfaces of the scaffolds to form tenocyte-scaffold constructs. The constructs were cryopreserved in a vitreous cryoprotectant (CPA) with a multi-step protocol. The cell adhesion to scaffolds was observed with electronic scanning microscopy (SEM). The elongation index of the living tenocytes and ratio of live/dead cell number were examined based on a live/dead dual fluorescent staining technique, and the survival rate of tenocytes was studied with flow cytometry (FC). The results showed the shapes of tenocytes varied between the different groups: flat or polygonal (on smooth surface), spindle (on micro-grooved surface), and spindle or ellipse (on porous surface). After thawing, the porous surface got the most living tenocytes and a higher survival rate, suggesting its potential application for vitreous cryopreservation of engineered tendon constructs.
URI: http://hdl.handle.net/10397/66475
ISSN: 0169-4332
EISSN: 1873-5584
DOI: 10.1016/j.apsusc.2016.01.187
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