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Title: Dielectrophoresis-induced cell patterning using a new PLA scaffold made by 3D printing
Authors: Huan, Z
Chu, HK 
Yang, J
Sun, D
Issue Date: 2016
Publisher: Institute of Electrical and Electronics Engineers Inc.
Source: 16th International Conference on Nanotechnology - IEEE NANO 2016, 2016, 7751549, p. 853-856 How to cite?
Abstract: This paper presents a new technique of fabricating 3D-printed scaffolds that can utilizes dielectrophoresis (DEP) for cell patterning. The scaffold was first fabricated using a 3D printer with a biodegradable polymer, polylactic acid (PLA). The electrical conductivity of the polymeric scaffold was enhanced through sputtering a thin layer of gold. When a voltage was supplied to the scaffold, non-uniform electric fields were generated so that cells were polarized and patterned onto the scaffold. Experiments were conducted to demonstrate that the gold-coated PLA scaffold could be used for rapid patterning MC3T3-E1 cells via DEP. Cell proliferation was assessed by (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT method and the result confirms the DEP cell patterning mechanism is not cytotoxic.
Description: 16th IEEE International Conference on Nanotechnology - IEEE NANO 2016, Japan, 22-25 August 2016
ISBN: 9781509039142
DOI: 10.1109/NANO.2016.7751549
Appears in Collections:Conference Paper

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