Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/66088
Title: Inhibition of AGEs/RAGE/Rho/ROCK pathway suppresses non-specific neuroinflammation by regulating BV2 microglial M1/M2 polarization through the NF-κB pathway
Authors: Chen, J
Sun, Z
Jin, M
Tu, Y
Wang, S
Yang, X
Chen, Q
Zhang, X
Han, Y
Pi, R
Keywords: Advanced glycation end products
Alzheimer's disease
BV2 cells
Neuroinflammation
Rho kinase
Issue Date: 2017
Publisher: Elsevier
Source: Journal of neuroimmunology, 2017, v. 305, p. 108-114 How to cite?
Journal: Journal of neuroimmunology 
Abstract: The microglia-mediated neuroinflammation plays an important role in the pathogenesis of Alzheimer's disease (AD). Advanced glycation end products (AGEs)/receptor for advanced glycation end products (RAGE) or Rho/Rho kinase (ROCK) are both involved in the development of non-specific inflammation. However, there are few reports about their effects on neuroinflammation. Here, we explored the mechanism of AGEs/RAGE/Rho/ROCK pathway underlying the non-specific inflammation and microglial polarization in BV2 cells. AGEs could activate ROCK pathway in a concentration-dependent manner. ROCK inhibitor fasudil and RAGE-specific blocker FPS-ZM1 significantly inhibited AGEs-mediated activation of BV2 cells and induction of reactive oxygen species (ROS). FPS-ZM1 and fasudil exerted their anti-inflammatory effects by downregulating inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), NLRP3 and nuclear translocation of nuclear factor kappa B (NF-κB) p65. In addition, AGEs induced both M1 (CD16/32, M1 marker) and M2 (CD206, M2 marker) phenotype in BV2 cells. Fasudil and FPS-ZM1 led to a decreased M1 and increased M2 phenotype. Together, these results indicate that the AGEs/RAGE/Rho/ROCK pathway in BV2 cells could intensify the non-specific inflammation of AD, which will provide novel strategies for the development of anti-AD drugs.
URI: http://hdl.handle.net/10397/66088
ISSN: 0165-5728
EISSN: 1872-8421
DOI: 10.1016/j.jneuroim.2017.02.010
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