Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/63754
Title: Production of poly-3-hydroxybutyrate by Bacillus sp. JMa5 cultivated in molasses media
Authors: Wu, Q
Huang, HH
Hu, GH
Chen, JC
Ho, KP
Chen, GQ
Keywords: PHB
Poly-3-hydroxybutyrate
Bacillus
Polyhydroxyalkanoates
PHA
Issue Date: 2001
Publisher: Springer Netherlands
Source: Antonie van leeuwenhoek, 2001, v. 80, no. 2, p. 111-118 How to cite?
Journal: Antonie van leeuwenhoek 
Abstract: A strain of Bacillus sp. coded JMa5 was isolated from molasses contaminated soil. The strain was able to grow at a temperature as high as 45°C and in 250 g/l molasses although the optimal growth temperature was 35–37°C. Cell density reached 30 g/l 8 h after inoculation in a batch culture with an initial concentration of 210 g/l molasses. Under fed-batch conditions, the cells grew to a dry weight of 70 g/l after 30 h of fermentation. The strain accumulated 25–35%, (w/w) polyhydroxybutyrate (PHB) during fermentation. PHB accumulation was a growth-associated process. Factors that normally promote PHB production include high ratios of carbon to nitrogen, and carbon to phosphorus in growth media. Low dissolved oxygen supply resulted in sporulation, which reduced PHB contents and dry weights of the cells. It seems that sporulation induced by reduced supply of nutrients is the reason that PHB content is generally low in the Bacillus strain.
URI: http://hdl.handle.net/10397/63754
ISSN: 0003-6072
EISSN: 1572-9699
DOI: 10.1023/A:1012222625201
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