Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/62419
DC Field | Value | Language |
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dc.contributor | Department of Applied Biology and Chemical Technology | - |
dc.creator | Sun, HY | - |
dc.creator | Liu, BB | - |
dc.creator | Hu, JY | - |
dc.creator | Xu, LJ | - |
dc.creator | Chan, SW | - |
dc.creator | Chan, CO | - |
dc.creator | Mok, DKW | - |
dc.creator | Zhang, DM | - |
dc.creator | Ye, WC | - |
dc.creator | Chen, SB | - |
dc.date.accessioned | 2016-12-19T09:00:38Z | - |
dc.date.available | 2016-12-19T09:00:38Z | - |
dc.identifier.issn | 1749-8546 (online) | - |
dc.identifier.uri | http://hdl.handle.net/10397/62419 | - |
dc.language.iso | en | en_US |
dc.publisher | BioMed Central | en_US |
dc.rights | © 2016 Sun etal. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en_US |
dc.rights | The following publication Sun, H. Y., Liu, B. B., Hu, J. Y., Xu, L. J., Chan, S. W., Chan, C. O., … Chen, S. B. (2016). Novel cycloartane triterpenoid from cimicifuga foetida (Sheng Ma) induces mitochondrial apoptosis via inhibiting RAF/MEK/ERK pathway and AKT phosphorylation in human breast carcinoma MCF-7 cells. Chinese Medicine, 11, 1, 1-11 is available at https://dx.doi.org/10.1186/s13020-015-0073-6 | en_US |
dc.title | Novel cycloartane triterpenoid from cimicifuga foetida (Sheng Ma) induces mitochondrial apoptosis via inhibiting RAF/MEK/ERK pathway and AKT phosphorylation in human breast carcinoma MCF-7 cells | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.identifier.epage | 11 | - |
dc.identifier.volume | 11 | - |
dc.identifier.doi | 10.1186/s13020-015-0073-6 | - |
dcterms.abstract | Background: Cycloartane triterpenoids exhibited anticancer effects. This study aims to identify any potential novel anticancer cycloartane triterpenoids from Cimicifuga foetida L. rhizome (Sheng ma) and the mode of actions. | - |
dcterms.abstract | Methods: Cycloartane triterpenoids were isolated from the C. foetida rhizome by a series of column chromatography and identified by IR, MS and NMR. Their anticancer effects on several human cancer cell lines, MCF-7, HepG2, HepG2/ADM, HeLa, and PC3, and normal human mammary epithelial cells MCF10A were investigated by colony formation and MTT assays. Morphological analysis of apoptosis induction was performed by acridine orange/ethidium bromide dual-staining and Hoechst 33258 nuclear staining. The cell-cycle profile and annexin V staining were evaluated by flow cytometry. Apoptosis were investigated by measuring changes in mitochondrial membrane potential and analyzing expression of cell cycle-and apoptosis-related proteins in MCF-7 cells by Western blotting. | - |
dcterms.abstract | Results: A novel cycloartane triterpenoid, 25-O-acetyl-7,8-didehydrocimigenol-3-O-beta-d-(2-acetyl) xylopyranoside (ADHC-AXpn), together with the known 7,8-didehydrocimigenol-3-O-beta-d-xylopyranoside (DHC-Xpn) were isolated. MCF-7 growth was significantly inhibited by ADHC-AXpn in a dose-and time-dependent manner (IC50: 27.81 mu M at 48 h; P = 0.004 vs. control at 25 mu M for 48 h treatment), and ADHC-AXpn was selectively cytotoxic for cancerous cells (MCF-7, HepG2/ADM, HepG2 and HELA cells) based on its higher IC50 values for normal cells MCF10A (IC50: 78.63 mu M at 48 h) than for tumor cells. In MCF-7 cells, ADHC-AXpn induced G2/M cell cycle arrest by mediating cyclin-B1, and CDK1 and its phosphorylation; and induced apoptosis through the mitochondrial-mediated apoptotic pathway, with inhibition of Akt activation. As ADHC-AXpn suppressed phosphorylation of ERK1/2, Raf and Akt proteins in MCF-7 cells, its apoptotic effect might be associated with Raf/MEK/ERK signaling and Akt activation. | - |
dcterms.abstract | Conclusions: ADHC-AXpn significantly suppressed the growth of MCF-7 cells, induced mitochondrial apoptosis and cell-cycle arrest, and inhibited Raf/MEK/ERK signaling pathway and Akt phosphorylation. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | Chinese medicine, 2016, v. 11, 1, p. 1-11 | - |
dcterms.isPartOf | Chinese medicine | - |
dcterms.issued | 2016 | - |
dc.identifier.isi | WOS:000367808400001 | - |
dc.identifier.pmid | 26759603 | - |
dc.identifier.artn | 1 | - |
dc.identifier.rosgroupid | 2015003166 | - |
dc.description.ros | 2015-2016 > Academic research: refereed > Publication in refereed journal | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
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Sun_Novel_Cycloartane_Triterpenoid.pdf | 6.1 MB | Adobe PDF | View/Open |
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