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Title: Effect of hedyotis diffusa water extract on protecting human hepatocyte cells (LO2) from H2O2-induced cytotoxicity
Authors: Gao, X
Li, C
Tang, YL
Zhang, H
Chan, SW
Keywords: Apoptosis
Liver disease
Reactive oxygen species
Traditional Chinese medicinal herb
Issue Date: 2016
Publisher: Taylor & Francis
Source: Pharmaceutical biology, 2016, v. 54, no. 7, p. 1148-1155 How to cite?
Journal: Pharmaceutical biology 
Abstract: Context: Natural products are good sources of natural dietary antioxidants that are believed to protect the body against hepatotoxic effect induced by oxidative stress. Hedyotis diffusa Willd (Rubiaceae) (HDW) is a traditional Chinese medicinal herb that has been shown to possess a variety of antioxidant properties.
Objective: The present study examines and explains the cell protective property of HDW water extract (WEHDW). Materials and methods: 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay was used to measure the free radical scavenging property of WEHDW (0.001–10 mg/mL). The protective effect of WEHDW (0.3–10 mg/mL 2 h pretreatment) against hydrogen peroxide (H2O2, 200 μM for 6 h) induced cytotoxicity in human hepatic cells, LO2, was evaluated using cell viability assay and nuclear staining. The molecular pathway of WEHDW's effect was investigated by using Western blot assay.
Results: WEHDW had a 50% scavenging concentration (SC50) at 0.153 mg/mL in the DPPH assay. Exposure of LO2 cells to H2O2 resulted in apoptosis which could be markedly attenuated by pre-treating WEHDW in a concentration-dependent manner (0.5, 1, 3, 5, or 10 mg/mL) (all with p < 0.001, versus control). Moreover, Hoechst (nuclear) staining showed that 1 mg/mL WEHDW could protect LO2 cells by attenuating apoptotic cell death mediated by H2O2. It was found that WEHDW reversed H2O2-induced activation of MEK/ERK pathway and H2O2-induced inhibition of P13-K/AKT/GSK3β pathway in LO2 cells.
Discussion and conclusion: WEHDW may help to improve the antioxidant defense system, resulting in prevention of oxidative stress-related fatty liver diseases.
ISSN: 1388-0209
EISSN: 1744-5116
DOI: 10.3109/13880209.2015.1056310
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