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Title: A high-saturated-fat, high-sucrose diet aggravates bone loss in ovariectomized female rats
Authors: Dong, XL
Li, CM
Cao, SS
Zhou, LP
Wong, MS 
Keywords: Bone loss
Estrogen deficiency
High-saturated-fat diet
High-sucrose diet
Oxidative stress
Issue Date: 2016
Publisher: American Society for Nutritional Sciences
Source: Journal of nutrition, 2016, v. 146, no. 6, p. 1172-1179 How to cite?
Journal: Journal of nutrition 
Abstract: Background: Estrogen deficiency in women and high-saturated fat, high-sucrose (HFS) diets have both been recognized as risk factors for metabolic syndrome. Studies on the combined actions of these 2 detrimental factors on the bone in females are limited. Objective: We sought to determine the interactive actions of estrogen deficiency and an HFS diet on bone properties and to investigate the underlying mechanisms.
Methods: Six-month-old Sprague Dawley sham or ovariectomized (OVX) rats were pair fed the same amount of either a low-saturated-fat, low-sucrose (LFS) diet (13% fat calories; 15% sucrose calories) or an HFS diet (42% fat calories; 30% sucrose calories) for 12 wk. Blood, liver, and bone were collected for correspondent parameters measurement.
Results: Ovariectomy decreased bone mineral density in the tibia head (TH) by 62% and the femoral end (FE) by 49% (P < 0.0001). The HFS diet aggravated bone loss in OVX rats by an additional 41% in the TH and 37% in the FE (P < 0.05). Bone loss in the HFS-OVX rats was accompanied by increased urinary deoxypyridinoline concentrations by 28% (P < 0.05). The HFS diet induced cathepsin K by 145% but reduced osteoprotegerin mRNA expression at the FE of the HFS-sham rats by 71% (P < 0.05). Ovariectomy significantly increased peroxisome proliferator-activated receptor g mRNA expression by 136% and 170% at the FE of the LFS- and HFS-OVX rats, respectively (P < 0.05). The HFS diet aggravated ovariectomyinduced lipid deposition and oxidative stress (OS) in rat livers (P < 0.05). Trabecular bone mineral density at the FE was negatively correlated with rat liver malondialdehyde concentrations (R2 = 0.39; P < 0.01).
Conclusions: The detrimental actions of the HFS diet and ovariectomy on bone properties in rats occurredmainly in cancellous bones and were characterized by a high degree of bone resorption and alterations in OS. J Nutr 2016;146:1172-9.
ISSN: 0022-3166 (print)
1541-6100 (online)
DOI: 10.3945/jn.115.225474
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