Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/61196
Title: Temperature induced modulation of lipid oxidation and lipid accumulation in palmitate-mediated 3T3-L1 adipocytes and 3T3-L1 adipocytes
Authors: Lin, X
Li, Y 
Leung, PH 
Li, J
Hu, J 
Liu, X
Li, Z
Keywords: Adipocytes
Cell viability
Lipid droplet size
Oxidative stress
Temperature
Issue Date: 2016
Publisher: Pergamon Press
Source: Journal of thermal biology, 2016, v. 58, p. 1-7 How to cite?
Journal: Journal of thermal biology 
Abstract: Human skin temperature can vary widely depending on anatomical location and ambient temperature. It is also known that local changes in skin and subcutaneous temperature can affect fat metabolism. This study aimed to explore the potential effects of surrounding thermal environment on fat by investigating cell viability, lipid oxidation, and lipid accumulation in 3T3-L1 adipocytes and palmitate-treated adipocytes after 4 h incubation. No significant differences of viability in 3T3-L1 adipocytes were detected under different temperature conditions. Despite no significant increase being observed under warm temperature (39 °C) conditions, a similarly significant suppression of intracellular reactive oxygen species (ROS) and lipid peroxidation were found in 3T3-L1 adipocytes and palmitate-treated adipocytes under 4 h exposure to cooler temperatures of 31-33 °C (P<0.01). ROS, chemically reactive molecules containing oxygen, are currently understood to be a major contributor to oxidantive stress in obesity. Additionally, cooler temperatures (31-33 °C) could improve the size of lipid droplets in 3T3-L1 adipocytes (P<0.01), but no significant effect was generated by temperature change on lipid droplets in palmitate-treated adipocytes. In the palmitate-induced adiposity model, although excessive ROS and lipid peroxidation has been attenuated by temperature decrease (P<0.01), it still does not positively modulate lipid droplet size (P>0.05) and remedy the palmitate damage induced cell death (P<0.01). These findings provide preliminary support for potential interventions based on temperature manipulation for cell metabolism of adipocytes.
URI: http://hdl.handle.net/10397/61196
ISSN: 0306-4565
DOI: 10.1016/j.jtherbio.2016.03.008
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