Please use this identifier to cite or link to this item:
http://hdl.handle.net/10397/5857
DC Field | Value | Language |
---|---|---|
dc.contributor | Department of Applied Social Sciences | - |
dc.creator | Seto, SW | - |
dc.creator | Au, ALS | - |
dc.creator | Poon, CCW | - |
dc.creator | Zhang, Q | - |
dc.creator | Li, RWS | - |
dc.creator | Yeung, JHK | - |
dc.creator | Kong, SK | - |
dc.creator | Ngai, SM | - |
dc.creator | Wan, S | - |
dc.creator | Ho, HP | - |
dc.creator | Lee, SMY | - |
dc.creator | Hoi, MPM | - |
dc.creator | Chan, SW | - |
dc.creator | Leung, GPH | - |
dc.creator | Kwan, YW | - |
dc.date.accessioned | 2014-12-11T08:24:03Z | - |
dc.date.available | 2014-12-11T08:24:03Z | - |
dc.identifier.uri | http://hdl.handle.net/10397/5857 | - |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science | en_US |
dc.rights | © 2013 Seto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.title | Acute simvastatin inhibits K[sub ATP] channels of porcine coronary artery myocytes | en_US |
dc.type | Journal/Magazine Article | en_US |
dc.description.otherinformation | Author name used in this publication: Simon Ming Yuen Lee | en_US |
dc.description.otherinformation | Author name used in this publication: George Pak Heng Leung | en_US |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 16 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 6 | - |
dc.identifier.doi | 10.1371/journal.pone.0066404 | - |
dcterms.abstract | Background: Statins (3-hydroxy-3-methyl-glutaryl coenzyme A (HMG-CoA) reductase inhibitors) consumption provides beneficial effects on cardiovascular systems. However, effects of statins on vascular K[sub ATP] channel gatings are unknown. | - |
dcterms.abstract | Methods: Pig left anterior descending coronary artery and human left internal mammary artery were isolated and endothelium-denuded for tension measurements and Western immunoblots. Enzymatically-dissociated/cultured arterial myocytes were used for patch-clamp electrophysiological studies and for [Ca²⁺][sub i], [ATP][sub i] and [glucose][sub o] uptake measurements. | - |
dcterms.abstract | Results: The cromakalim (10 nM to 10 µM)- and pinacidil (10 nM to 10 µM)-induced concentration-dependent relaxation of porcine coronary artery was inhibited by simvastatin (3 and 10 µM). Simvastatin (1, 3 and 10 µM) suppressed (in okadaic acid (10 nM)-sensitive manner) cromakalim (10 µM)- and pinacidil (10 µM)-mediated opening of whole-cell KATP channels of arterial myocytes. Simvastatin (10 µM) and AICAR (1 mM) elicited a time-dependent, compound C (1 µM)-sensitive [³H]-2-deoxy-glucose uptake and an increase in [ATP][sub i] levels. A time (2–30 min)- and concentration (0.1–10 µM)-dependent increase by simvastatin of p-AMPKα-Thr¹⁷² and p-PP2A-Tyr³⁰⁷ expression was observed. The enhanced p-AMPKα-Thr¹⁷² expression was inhibited by compound C, ryanodine (100 µM) and KN93 (10 µM). Simvastatin-induced p-PP2A-Tyr³⁰⁷ expression was suppressed by okadaic acid, compound C, ryanodine, KN93, phloridzin (1 mM), ouabain (10 µM), and in [glucose][sub o]-free or [Na⁺][sub o]-free conditions. | - |
dcterms.abstract | Conclusions: Simvastatin causes ryanodine-sensitive Ca²⁺ release which is important for AMPKα-Thr¹⁷² phosphorylation via Ca²⁺/CaMK II. AMPKα-Thr¹⁷² phosphorylation causes [glucose]o uptake (and an [ATP]i increase), closure of KATP channels, and phosphorylation of AMPKα-Thr¹⁷² and PP2A-Tyr³⁰⁷ resulted. Phosphorylation of PP2A-Tyr³⁰⁷ occurs at a site downstream of AMPKα-Thr¹⁷² phosphorylation. | - |
dcterms.accessRights | open access | en_US |
dcterms.bibliographicCitation | PLoS one, 17 June 2013, v. 8, no. 6, e66404, p. 1-16 | - |
dcterms.isPartOf | PLoS one | - |
dcterms.issued | 2013-06-17 | - |
dc.identifier.isi | WOS:000321397800030 | - |
dc.identifier.scopus | 2-s2.0-84879188037 | - |
dc.identifier.pmid | 23799098 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.rosgroupid | r62173 | - |
dc.description.ros | 2012-2013 > Academic research: refereed > Publication in refereed journal | - |
dc.description.oa | Version of Record | en_US |
dc.identifier.FolderNumber | OA_IR/PIRA | en_US |
dc.description.pubStatus | Published | en_US |
Appears in Collections: | Journal/Magazine Article |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Seto_Acute_Simvastatin_KATP.pdf | 3.29 MB | Adobe PDF | View/Open |
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