Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/55730
Title: Assessment of membrane protection by traditional Chinese medicines using a flow cytometric technique : preliminary findings
Authors: Chung, WY
Yow, CMN
Benzie, IFF 
Issue Date: 2003
Publisher: Maney Publishing
Source: Redox report, 2003, v. 8 , no. 1, p. 31-33 How to cite?
Journal: Redox report 
Abstract: In this preliminary study, we used a 'living cell' flow-cytometric approach to membrane protection by four traditional Chinese medicines (TCMs). Cells were incubated, separately, for 30 min with aqueous extracts (1.5% w/v) of lingzhi (Ganoderma lucidum), ginger (Zingiber officianale), ginseng (Panax ginseng), and green tea (Camellia sinensis). Membranes were labelled with a fluorescent probe, cells were then incubated with cumene hydroperoxide, and site-specific oxidation induced by iron/ascorbate. Oxidation of membrane lipids quenches fluorescence. Forward-scatter fluorescence was measured at timed intervals after initiation of oxidation. Results indicate that lingzhi and ginger contain antioxidant component(s) that act within the cell membrane and slow lipid peroxidation in situ. Results demonstrate also that this living cell model is a useful biomonitoring tool to help determine molecular aspects of putative health effects of TCMs.
URI: http://hdl.handle.net/10397/55730
ISSN: 1351-0002 (print)
1743-2928 (online)
DOI: 10.1179/135100003125001215
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