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Title: Extracellular ATP-induced NO production and its dependence on membrane Ca2+ flux in Salvia miltiorrhiza hairy roots
Authors: Wu, SJ
Wu, JY 
Keywords: Ca2+
Extracellular ATP
Hairy roots
Nitric oxide
Reactive oxygen species
Salvia miltiorrhiza
Issue Date: 2008
Publisher: Oxford University Press
Source: Journal of experimental botany, 2008, v. 59 , no. 14, p. 4007-4016 How to cite?
Journal: Journal of experimental botany 
Abstract: Extracellular ATP (eATP) is a novel signalling agent, and nitric oxide (NO) is a well-established signal molecule with diverse functions in plant growth and development. This study characterizes NO production induced by exogenous ATP and examines its relationship with other important signalling agents, Ca 2+ and H2O2 in Salvia miltiorrhiza hairy root culture. Exogenous ATP was applied at 10-500 μM to the hairy root cultures and stimulated NO production was detectable within 30 min. The NO level increased with ATP dose from 10-100 μM but decreased from 100-200 μM or higher. The ATP-induced NO production was mimicked by a non-hydrolysable ATP analogue ATPγS, but only weakly by ADP, AMP or adenosine. The ATP-induced NO production was blocked by Ca2+ antagonists, but not affected by a protein kinase inhibitor. ATP also induced H2O2 production, which was dependent on both Ca2+ and protein kinases, and also on NO biosynthesis. On the other hand, ATP induced a rapid increase in the intracellular Ca2+ level, which was dependent on NO but not H 2O2. The results suggest that NO is implicated in ATP-induced responses and signal transduction in plant cells, and ATP signalling is closely related to Ca2+ and ROS signalling.
ISSN: 0022-0957 (print)
1460-2431 (online)
DOI: 10.1093/jxb/ern242
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