Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/55641
Title: Construction of recombinant Escherichia coli strains for polyhydroxybutyrate production using soy waste as nutrient
Authors: Hong, K
Chua, H
Leung, YC 
Kwok, SY
Law, KH
Yu, PHF
Keywords: Escherichia coli
Fourier- transform infrared
Lac promotor
Polyhydroxybutyrates
Soy waste
T7 promotor
Issue Date: 2000
Publisher: Humana Press
Source: Applied biochemistry and biotechnology, 2000, v. 84-86, p. 381-390 How to cite?
Journal: Applied biochemistry and biotechnology 
Abstract: Construction and comparison of recombinant Escherichia coli strains harboring the polyhydroxybutyrate (PHB) operon from Ralstonia eutropha using vectors possessing different promotors, as well as the production of PHB from soy waste by the recombinant strain, are reported. The lac promotor was the most efficient on expression of the phb operon among the three promotors studied: i.e., lac promotor, T7 promotor and the normal σ70 promotor. The pKS/PHB was the most efficient plasmid for phb operon expression among the three plasmids used: i.e., pKS-, pAED4, and pJM9131. It was observed that isopropyl-β-D-thiogalactopyanoside was not required for the induction of the expression of phb operon. The cell dry wt and polyhydroxyalkanoate content by E. coli XL-1 Blue (pKS/PHB) were 3.025 g/L and 27.83%, respectively.
URI: http://hdl.handle.net/10397/55641
ISSN: 0273-2289
EISSN: 1559-0291
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