Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/55479
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dc.contributorDepartment of Applied Biology and Chemical Technology-
dc.creatorLi, S-
dc.creatorDang, Y-
dc.creatorZhou, X-
dc.creatorHuang, B-
dc.creatorHuang, X-
dc.creatorZhang, Z-
dc.creatorKwan, YW-
dc.creatorChan, SW-
dc.creatorLeung, GPH-
dc.creatorLee, SMY-
dc.creatorHoi, MPM-
dc.date.accessioned2016-09-07T02:21:59Z-
dc.date.available2016-09-07T02:21:59Z-
dc.identifier.urihttp://hdl.handle.net/10397/55479-
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en_US
dc.rightsThe following publication Li, S. et al. Formononetin promotes angiogenesis through the estrogen receptor alpha-enhanced ROCK pathway. Sci. Rep. 5, 16815 (2015) is available at https://dx.doi.org/10.1038/srep16815en_US
dc.titleFormononetin promotes angiogenesis through the estrogen receptor alpha-enhanced ROCK pathwayen_US
dc.typeJournal/Magazine Articleen_US
dc.identifier.volume5-
dc.identifier.doi10.1038/srep16815-
dcterms.abstractFormononetin is an isoflavone that has been shown to display estrogenic properties and induce angiogenesis activities. However, the interrelationship between the estrogenic properties and angiogenesis activities of formononetin are not well defined. In the present study, docking and enzymatic assay demonstrated that formononetin displayed direct binding to the ligand-binding domain (LBD) of estrogen receptor alpha (ERα) with an agonistic property. Results from Human Umbilical Vein Endothelial Cells (HUVEC) by using real-time migration xCELLigence system, immunofluorescence and western blotting provided strong evidences of formononetin induced endothelial cell migration and dramatic actin cytoskeleton spatial modification through ERα-enhanced-ROCK-II/MMP2/9 signaling pathways. In addition, results from co-immunoprecipitation suggested formononetin induced cell migration via recruiting of ERα/ROCK-II activated complex formation. More interestingly, in zebrafish embryo we observed that formononetin significantly promoted angiogenic sproutings in the subintestinal vessels (SIVs) that could be completely abolished by ROCK inhibitor. In this study, we elucidated the underlying mechanisms that formononetin produced proangiogenesis effects through an ERα-enhanced ROCK-II signaling pathways. Results from the present study also expand our knowledge about the enigmatic underlying mechanisms of phytoestrogenic compounds in the promotion of angiogenesis in relation to ERα and ROCK interaction in endothelial cells and their relationship with actin assembly and cell migration.-
dcterms.accessRightsopen accessen_US
dcterms.bibliographicCitationScientific reports, 16 2015, v. 5, no. , p. 1-17-
dcterms.isPartOfScientific reports-
dcterms.issued2015-
dc.identifier.scopus2-s2.0-84947256819-
dc.identifier.eissn2045-2322-
dc.identifier.rosgroupid2015003167-
dc.description.ros2015-2016 > Academic research: refereed > Publication in refereed journal-
dc.description.oaVersion of Recorden_US
dc.identifier.FolderNumberOA_IR/PIRAen_US
dc.description.pubStatusPublisheden_US
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