Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/5223
Title: Total flavonoid fraction of rhizoma drynaria improves bone properties in ovariectomized mice and exerts estrogen-like activities in rat osteoblast-like (UMR-106) cells
Authors: Pang, WY
Wang, XL
Wong, KC
Leung, PC
Yao, XS
Wong, MS 
Issue Date: 2012
Publisher: Food and Drug Administration, Department of Health, Executive Yuan
Source: Journal of food and drug analysis, 2012, v. 20, suppl. 1, p. 265-269 How to cite?
Journal: Journal of food and drug analysis 
Abstract: Rhizoma Drynaria (RD) has been widely used for healing bone fractures or related diseases in traditional Chinese medicine. The present study was designed to determine if RD total flavonoids (RDTF) could exert estrogen-like protective actions on bone. Young C57/BL6J mice were ovariectomized (OVX) and treated orally with RDTF (0.087, 0.173 or 0.346 mg/g/day), 17β-estradiol (2μg/g/day) or its vehicle for 6 weeks. Bone mineral densities (BMD) was measured by peripheral quantitative computed tomography (pQCT). Rat osteoblast-like UMR-106 cells were co-incubated with ER antagonist ICI 182, 780 to determine if the effects of RDTF on osteoblastic functions were ER-dependent. The functional transactivation of ER-α and ER-β as well as ER-αphosphorylation by RDTF were also studied. RDTF increased trabecular-rich BMD at distal femur and lumbar spine in OVX mice. 0.173 mg/g/day was most effective in improving bone properties in OVX mice. The stimulatory effects of RDTF on osteoblastic functions could be abolished by co-incubation with ICI 182, 780 in UMR-106 cells. Transient transfection study indicated that RDTF dose-dependently stimulated ERE-dependent luciferase activity in UMR-106 cells via ER-α and ER-β. Moreover, 0.2 μg/mL of RDTF significantly induced ER-α phosphorylation at serine 118 in UMR-106 cells.
URI: http://hdl.handle.net/10397/5223
ISSN: 1738-3838
Rights: Posted with permission of the publisher.
Appears in Collections:Journal/Magazine Article

Files in This Item:
File Description SizeFormat 
20_S1__P265-269.pdf514.5 kBAdobe PDFView/Open
Access
View full-text via PolyU eLinks SFX Query
Show full item record

SCOPUSTM   
Citations

3
Last Week
0
Last month
1
Citations as of Apr 10, 2016

WEB OF SCIENCETM
Citations

2
Last Week
0
Last month
0
Citations as of May 2, 2016

Page view(s)

284
Last Week
0
Last month
Checked on May 1, 2016

Download(s)

233
Checked on May 1, 2016

Google ScholarTM

Check



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.