Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/43531
Title: In vitro Vitamin K2 and 1α,25-dihydroxyvitamin D3 combination enhances osteoblasts anabolism of diabetic mice
Authors: Poon, CCW
Li, RWS
Seto, SW
Kong, SK
Ho, HP
Hoi, MPM
Lee, SMY
Ngai, SM
Chan, SW
Leung, GPH
Kwan, YW
Keywords: 1,25(OH)2D3
Osteoblasts
Osteoporosis
Type 2 diabetes mellitus
Vitamin K2
Issue Date: 2015
Publisher: Elsevier
Source: European journal of pharmacology, 2015, v. 767, p. 30-40 How to cite?
Journal: European journal of pharmacology 
Abstract: In this study, we evaluated the anabolic effect and the underlying cellular mechanisms involved of vitamin K2 (10 nM) and 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) (10 nM), alone and in combination, on primary osteoblasts harvested from the iliac crests of C57BL/KsJ lean (+/+) and obese/diabetic (db/db) mice. A lower alkaline phosphatase (ALP) activity plus a reduced expression of bone anabolic markers and bone formation transcription factors (osteocalcin, Runx2, Dlx5, ATF4 and OSX) were consistently detected in osteoblasts of db/db mice compared to lean mice. A significantly higher calcium deposits formation in osteoblasts was observed in lean mice when compared to db/db mice. Co-administration of vitamin K2 (10 nM) and 1,25(OH)2D3 (10 nM) caused an enhancement of calcium deposits in osteoblasts in both strains of mice. Vitamins K2 and 1,25(OH)2D3 co-administration time-dependently (7, 14 and 21 days) increased the levels of bone anabolic markers and bone formation transcription factors, with a greater magnitude of increase observed in osteoblasts of db/db mice. Combined vitamins K2 plus 1,25(OH)2D3 treatment significantly enhanced migration and the re-appearance of surface microvilli and ruffles of osteoblasts of db/db mice. Thus, our results illustrate that vitamins K2 plus D3 combination could be a novel therapeutic strategy in treating diabetes-associated osteoporosis.
URI: http://hdl.handle.net/10397/43531
ISSN: 0014-2999
DOI: 10.1016/j.ejphar.2015.09.048
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