Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/43177
Title: Study of the efficacy and mechanism of ALA-mediated photodynamic therapy on human hepatocellular carcinoma cell
Authors: Yow, CMN
Wong, CK
Huang, Z
Ho, RJY
Keywords: ALA
Apoptosis
HepG2
P53
Photodynamic therapy
PpIX
Issue Date: 2007
Publisher: Wiley-Blackwell
Source: Liver international, 2007, v. 27, no. 2, p. 201-208 How to cite?
Journal: Liver international 
Abstract: Objectives: To examine the efficacy and mechanism of δ- or 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) on a human hepatocellular carcinoma cell line.
Materials and methods: The optimal uptake of photosensitizer ALA in HepG2 (p53 wild) cells was investigated by means of spectrometric measurement. Cell viability was determined by trypan blue exclusion assay. Morphological apoptotic changes in HepG2 cells before and after ALA-mediated PDT were determined by microscopic examination. Detection of apoptotic bodies was examined by DAPI staining. The changes in p53 expression were revealed by the immunostaining method.
Results: ALA/protoporphyrin IX (PpIX) was mainly located in the cytoplasm of HepG2 cells. The maximal cellular uptake occurred after 18 h in vitro incubation. The photocytotoxic assay showed that ALA PDT induced 80% killing at 2 mM drug dose and 2 J/cm2 light intensity. Up to 70% of cells showed membrane blebbing and positive DAPI staining, indicating that ALA–PDT-mediated cell death was predominantly via apoptosis. In addition, p53 was upregulated after treatment, implying that p53 might evoke apoptotic cell death.
Conclusions: HepG2 cell line is sensitive to ALA-mediated PDT. ALA–PDT induces apoptosis in the HepG2 cell line that may be mediated by a p53-dependent pathway.
URI: http://hdl.handle.net/10397/43177
ISSN: 1478-3223 (print)
1478-3231 (online)
DOI: 10.1111/j.1478-3231.2006.01412.x
Appears in Collections:Journal/Magazine Article

Access
View full-text via PolyU eLinks SFX Query
Show full item record

WEB OF SCIENCETM
Citations

36
Last Week
0
Last month
Citations as of Mar 21, 2017

Page view(s)

6
Last Week
0
Last month
Checked on Mar 19, 2017

Google ScholarTM

Check

Altmetric



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.