Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/43034
Title: Cellular uptake, subcellular localization and photodamaging effect of Temoporfin (mTHPC) in nasopharyngeal carcinoma cells : comparison with hematoporphyrin derivative
Authors: Yow, CMN
Chen, JY
Mak, NK
Cheung, NH
Leung, AWN
Keywords: Nasopharyngeal carcinoma
Photosensitizers
Photodynamic therapy
Issue Date: 2000
Publisher: Elsevier
Source: Cancer letters, 2000, v. 157, no. 2, p. 123-131 How to cite?
Journal: Cancer letters 
Abstract: Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than hematoporphyrin derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.
URI: http://hdl.handle.net/10397/43034
ISSN: 0304-3835
DOI: 10.1016/S0304-3835(00)00453-5
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