Please use this identifier to cite or link to this item:
Title: Effect of different durations of exercise on transferrin-bound iron uptake by rat erythroblast
Authors: Qian, ZM
Xiao, DS
Ke, Y
Tang, PL
Keywords: Transferrin-bound iron (Tf-Fe)
Transferrin (Tf) internalization
Transferrin receptor (TfR) expression
Iron deficiency
Length of strenuous exercise
Issue Date: 2002
Publisher: Elsevier
Source: Journal of nutritional biochemistry, 2002, v. 13, no. 1, p. 47-54 How to cite?
Journal: Journal of nutritional biochemistry 
Abstract: This study evaluated effects of different durations of exercise on transferrin receptor (TfR) expression on the membrane of rat erythroblasts. Female rats were assigned to six groups: 3, 6 and 12 months of strenuous exercise (swimming 2 h/day, 5 days/wk) groups and their corresponding controls. At the end of experiments, the erythroblasts were isolated for Tf binding assay and transferrin-bound iron (Tf-Fe) uptake. Tissue non-heme iron and hematological iron indices were also measured. The TfR number on the cells was about 603,189 ± 107,562, 890,150 ± 164,849 and 384,695 ± 46,295 molecules/cell in three control groups (3, 6, 12 months) respectively. Exercise groups had significantly higher levels of TfR than those of the control groups, being 1,374,137 ± 243,677, 2,175,360 ± 462,737 and 1,012,759 ± 249,423 molecules/cell in 3, 6 and 12 months of exercise groups respectively (p < 0.05). After 30 min of incubation, cellular Tf approached to levels of 8.28 ± 1.94, 10.73 ± 3.30 and 6.60 ± 0.93 fmole/106 cells in 3, 6 and 12 months of exercise groups, while the corresponding control values were 3.09 ± 0.36, 5.03 ± 1.01 and 2.51 ± 0.88 fmole/106 cells respectively (all P < 0.05). The rates of cellular iron accumulation were 7.07 (3), 8.79 (6) and 5.96 (12 month) fmole/106 cells/min in the exercised rats and 2.91, 3.85, and 2.03 fmole/106 cells/min in their corresponding controls (all p < 0.05). However, no significant difference was observed in the ratios (Exercise/Corresponding control) of the increased TfR expression, Tf-Fe uptake and Tf endocytosis as well as of the decreased plasma iron and tissue non-heme iron levels induced by different periods of exercise. Furthermore, the increase in the length of exercise (6 or 12 month) did not induce a remarkable decrease in plasma hemoglobin and hematocrit. These results indicate that a true iron deficiency or ‘sport anemia' can not develop even if under longer periods (6 or 12 month) of strenuous exercise.
ISSN: 0955-2863
DOI: 10.1016/S0955-2863(01)00194-2
Appears in Collections:Journal/Magazine Article

View full-text via PolyU eLinks SFX Query
Show full item record


Last Week
Last month
Citations as of Sep 15, 2018

Page view(s)

Last Week
Last month
Citations as of Sep 17, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.