Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/36313
Title: Non-contact high-frequency ultrasound microbeam stimulation for studying Mechanotransduction in human Umbilical Vein Endothelial cells
Authors: Hwang, JY
Lim, HG
Yoon, CW
Lam, KH 
Yoon, S
Lee, C
Chiu, CT
Kang, BJ
Kim, HH
Shung, KK
Keywords: High-frequency ultrasound microbeam
Mechanotransduction
Human umbilical vein endothelial cells
Calcium fluorescence imaging
Issue Date: 2014
Publisher: Elsevier
Source: Ultrasound in medicine and biology, 2014, v. 40, no. 9, p. 2172-2182 How to cite?
Journal: Ultrasound in medicine and biology 
Abstract: We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechano-transduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. Avariety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.
URI: http://hdl.handle.net/10397/36313
ISSN: 0301-5629
DOI: 10.1016/j.ultrasmedbio.2014.03.018
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