Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/36272
Title: Facile and effective separation of polysaccharides and proteins from Cordyceps Sinensis Mycelia by ionic liquid aqueous two-phase system
Authors: Yan, JK
Ma, HL
Pei, JJ
Wang, ZB
Wu, JY 
Keywords: Cordyceps sinensis
Polysaccharides
Ionic liquid
Aqueous two-phase system
Separation
Issue Date: 2014
Publisher: Pergamon Press
Source: Separation and purification technology, 2014, v. 135, p. 278-284 How to cite?
Journal: Separation and purification technology 
Abstract: An ionic liquid aqueous two-phase system (ILATPS) based on the hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride ([C(4)mim]Cl) and inorganic salts (K3PO4 and K2HPO4) was employed to separate Cordyceps sinensis polysaccharides (CSPS) and proteins by a single-step procedure. The maximum extraction efficiencies of CSPS and proteins were 89.4% and 88.2%, respectively, as obtained with the [C(4)mim]Cl/K3PO4 system at 25 degrees C and pH 13.0 under optimal conditions. The partially purified CSPS obtained from the bottom salt-rich phase comprised glucose, mannose, and galactose at a molar ratio of 10:3.5:1. The backbone of CSPS mainly comprised alpha-1,4-linked-D-glucose residues with a molecular weight of 2.3 x 10(7) Da. The results of this study suggest that ILATPS is an efficient and environmentally friendly system that will highlight novel possibilities in the large-scale separation and purification of active polysaccharides from fungi or mushrooms.
URI: http://hdl.handle.net/10397/36272
ISSN: 1383-5866
DOI: 10.1016/j.seppur.2014.03.020
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